Abstract
Saponins are widely distributed in plant kingdom. In view of their wide range of biological activities and occurrence as complex mixtures, saponins have been purified and separated by high-performance liquid chromatography using reverse-phase columns at lower wavelength. Mostly, saponins are not detected by ultraviolet detector due to lack of chromophores. Electrospray ionization mass spectrometry, diode array detector, evaporative light scattering detection, and charged aerosols have been used for overcoming the detection problem of saponins.
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Keywords
Charged aerosol
diode array detector
evaporative light scattering detection
ESI
high-performance liquid chromatography
mass spectrometry
mobile phase
potodiode array detectors
saponins
sapogenins
ultraviolet
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