Home | About us | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Reviews
Search Article 
Advanced search 

 Table of Contents  
Year : 2018  |  Volume : 12  |  Issue : 24  |  Page : 230-237  

Pharmacology of Mikania genus: A systematic review

1 Laboratory of Chromatography and Mass Spectrometry, Faculty of Pharmacy, Federal University of Pará, Belém, Pará, Brazil
2 School of Pharmacy, University of Missouri, Kansas City, Missouri, USA

Date of Web Publication12-Oct-2018

Correspondence Address:
Prof. Wagner Luiz Ramos Barbosa
Laboratory of Chromatography and Mass Spectrometry, Faculty of Pharmacy, Federal University of Pará, Augusto Corrêa St, 01, CEP 66.075-900- Belém, PA
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/phrev.phrev_10_18

Rights and Permissions

Genus Mikania, in which “guaco” species are included, encloses many species of pharmaceutical interest that are well distributed throughout South America. This work aims to make a systematic review of the clinical and nonclinical data already published about some Mikania species and their existing products, available in the pharmaceutical market. As usual, some species are more studied than others and the most studied species to date are Mikania glomerata, Mikania laevigata, Mikania scandens, and Mikania micrantha. The first two are widely used in Brazil to treat respiratory disorders and are available in different preparations marketed in retail pharmaceutical stores. Among the reported activities, anti-inflammatory, analgesic, antibacterial, and central nervous system activities were the most tested since they are directly related to the popular use of some species of this genus. In addition, a noteworthy amount of toxicological studies in animals are published in the literature. Thus, this systematic review aims to gather knowledge about Mikania genus and consequently to contribute for a safer use of derivatives of its species.

Keywords: Asteraceae, medicinal plants, pharmacological effects, phytotherapy

How to cite this article:
Brigida da Silva AS, Owiti AO, Barbosa WL. Pharmacology of Mikania genus: A systematic review. Phcog Rev 2018;12:230-7

How to cite this URL:
Brigida da Silva AS, Owiti AO, Barbosa WL. Pharmacology of Mikania genus: A systematic review. Phcog Rev [serial online] 2018 [cited 2019 Jul 20];12:230-7. Available from: http://www.phcogrev.com/text.asp?2018/12/24/230/243191

   Introduction Top

The World Health Organization (WHO), in 2013, elaborated the strategies aiming the official implantation of integrative and complementary practices (ICPs), which include phytotherapy (complementary traditional medicine), in the health system of WHO-members countries from different continents, from different ethnic and cultural origins, and where different ICPs are officialized, either financed by health insurance system, or inserted in public policies that mobilize important portions of each population, like practitioners or patients of the complementary traditional medicine. It is noteworthy to point that a complementary traditional medicine of quality, safety, and effectiveness contributes to the broad universal access to health care.[1]

Moreover, in the last decades, the use of medicinal plants has increased substantially, either as remedies used in the traditional medicine [2],[3] or as a material for producing dietary supplements, both in Western world and Asia. Despite the extensive development of pharmaceutical synthesis methods, medicinal plants still represent important sources of new products, mainly because plants can synthesize and produce constituents that are structurally too complex to be obtained through chemical synthesis.[3]

In the context of the phytotherapy, different botanical families contribute with many genus and, considering the genera from Asteraceae family, Mikania presents some species, popularly known as “guaco,” which have been widely used in Brazil due to their therapeutic properties. Species such as Mikania glomerata and Mikania laevigata are widely used to treat respiratory diseases on the basis of their antitussive, expectorant, and bronchodilator activities,[4] in the form of phytomedicines, either as magistral or officinal formulations.[5]

Mikania genus belongs to Asteraceae family, which includes about 22,800 species already described,[6] some of them are widely used in folk medicine. Approximately, 450 Mikania species are mainly distributed in the American tropical and temperate regions. In Brazil, 203 species are found.[7]

In South America, there are two major diversity centers of the genus; one located in Brazil, between Minas Gerais, Rio de Janeiro, Paraná, and Santa Catarina States, comprising approximately 170 species. There are also many species occurring in Paraguay, Uruguay, and Argentina. The other distribution center, with approximately 150 species, is in the Andean countries, from Colombia to Bolivia. A small number of Mikania species, one of the few Asteraceae genera that develops in the lowlands of the Amazon region, is found outside these centers, since this region is considered inadequate for most of the members of this family.[8]

Mikania species have acknowledged therapeutic importance since they show different pharmacological activities, according to the literature.[9] The therapeutic activities are highlighted as that on respiratory tract, anti-inflammatory,[10],[11],[12],[13],[14],[15] anti-allergic, analgesic,[16] antioxidant,[17] as well on the central nervous system (CNS)[18] and antimicrobial.[19]

The quantification of active substances present in medicinal plants is a way for guaranteeing the quality of their derivatives used in phytotherapeutical practice. In this sense, metabolites of different classes have already been isolated from Mikania species, which are usually associated with their pharmacological activities, the main ones are coumarin, o-coumaric acid, kaurenoic acid, cinnamoylgrandifloric acid, syringaldehyde, and stigmasterol or metabolic classes such as triterpenes/steroids, flavonoids, sesquiterpenes and lactones, saponins, and tannins. Caffeine derivatives among other substances have already been found in fewer amounts. In addition, other analyses revealed the presence of alcohols, acids, esters, aldehydes, and organic esters in Mikania species.[20]

Medicinal plants are subject of a very large number of scientific investigations, concerning their chemical composition or pharmacological properties, whether in animals and humans. In addition to generating data that support the development of new medicines, such works can also guarantee the safety and effectiveness of the use of these plant species in phytotherapy programs, as magistral or officinal formulations or industrial products. Studies focusing on Mikania species demonstrating the relation between pharmacological activity and chemical composition are more frequent than those reporting clinical studies of herbal derivatives from the genus, which are more important to guarantee the safety of a formulation. Therefore, this work presents a systematical review of Mikania genus, including nonclinical and clinical studies. The review involves studies already published on pharmacology, toxicology, and kinetics of samples prepared from Mikania species and aims to provide a reference base that contributes to build a wide scenario of the genus, which contributes for better understanding of their characteristics and for supporting the broad range of products and services based on popular phytotherapy.

   Materials and Methods Top

Survey tools, data selection, and organization

Databases queried and keywords used

A systematic review was performed consisting of a search in several databases such as Medline (by PubMed), ScienceDirect, Scopus, Lilacs, Scielo, Google Scholar, and Periodicos Capes without prior selection of dates to fetch all the articles of several years available until July 2017. The search was performed using the following keywords: “Mikania,” “toxicology,” “pharmacology,” “pharmacokinetic,” and “clinical” always using the strategy of combining them: “Mikania” and “pharmacology”, or “Mikania” and “pharmacokinetic,” or “Mikania” and “toxicology,” or “Mikania” and “clinical.”

Article selection criteria

Only scientific articles published in journals were selected. Theses, dissertations, and conference abstracts were not considered.

Organization of collected data

This work is organized in two sections containing results of experiments performed in nonclinical investigations, comprehending in vitro and in vivo experiments, and clinical studies. Each section comprises different activities assayed and reported in the selected articles.

   Results and Discussion Top

The systematic search in several databases allowed to observe that Google Scholar offers higher number of articles using “Mikania” as keyword, reaching around 16,700 titles (excluding citations), and Lilacs provided the smallest list of articles, according to [Figure 1]. The search has found no data on patents or books.
Figure 1: All results for word “Mikania

Click here to view

Performing an advanced search, associating keywords, the number of articles reduced significantly, as observed in [Table 1]. The base Google Scholar, again, showed higher number of references in all strategies. This occurs probably because the platform encompasses other databases. The results achieved using the combination “Mikania” and “pharmacology” provided the largest number of articles. Searching for clinical studies, however, it can be observed that the articles, in most databases, using the keyword “clinical,” do not differentiate tests by those in experimental animals or humans.
Table 1: Number of articles collected, according to the used keywords

Click here to view

The lowest number of articles was obtained using “Mikania” and “pharmacokinetic” as keywords providing 249 articles, which indicates that this topic needs to be further investigated for a better understanding of the kinetics of the substances present in the genus and thus bring up enough data to guarantee more safety for use.

Nonclinical studies

In vitro

Cytotoxicity and anticancer activities

Hexanic and ethanolic extracts from M. laevigata were tested for cytotoxicity against tumor and nontumor cells which presented inhibitory activity, these extracts show selectivity for both cell types.[21]

Sesquiterpene lactones isolated from Mikania micrantha have their anticancer activity evaluated in vitro on six human tumor cell lines and exhibited antiproliferative activity,[22] and the aqueous extract from the same species inhibited the activity of K562 and Hela cells in vitro and the growth of S180 sarcoma cells, in vivo, through multiple mechanisms including inhibition of proliferation, induction of apoptosis, and arrest of cell cycle, showing, additionally, low toxicity on immune system.[23]

The diterpene, ent-pimara-8,[14] 15-dien-19-oic acid, and three thymol derivatives, 10-acetoxy-8,9-dehydro-6-methoxythymol butyrate, 10-acetoxy-8,9-epoxy-6-methoxythymol isobutyrate, and acetylschizoginol from Mikania decora exhibited significant cytotoxic activity against a panel of human tumor cell lines.[24]

Anti-inflammatory activity

Anti-inflammatory activity is one of the most investigated aspects in Mikania genus. This activity of hydroalcoholic extracts of Mikania scandens roots and aerial parts was surveyed using the in vitro protein denaturation and the experiments indicated that the denaturation of the protein (albumin) was inhibited in a concentration-dependent way, for both extracts, but the root extract was more active.[25] Similar results were found by Banerjee et al., who investigated hydroalcoholic extracts from leaves and stem. The leaf extract exhibited a more potent anti-inflammatory in vitro activity by denaturation of protein (egg albumin) model, higher than the stem extract.[15]

For Mikania cordifolia, a phenolic constituent isolated from a leaf extract – 3,5-di-O-caffeoylquinic acid – showed an in vitro anti-inflammatory activity expressed as inhibition of monocyte migration and superoxide anion production.[26]

Antibacterial activity

The antibacterial activity of aqueous and methanolic extracts from M. micrantha was tested against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Proteus vulgaris, and Enterobacter aeruginosa; both the extracts were active against all microorganisms, except the latter.[27] From the same species, mikanolide and two derivatives were isolated from organic extract using chromatographic methods and were investigated for their antibacterial activity. The investigation revealed that the substances show antibacterial activity, but only pathogenic S. aureus and beta hemolytic Streptococcus Group A were susceptible at 100 μg of each substance per disc.[28]

The antibacterial activity of the isolated compounds from M. micrantha chloroform extract was evaluated, and all the isolated compounds (deoxymikanolide, scandenolide, dihydroscandenolide, mikanolide, dihydromikanolide, and m-methoxybenzoic acid) were effective against the tested strains. Deoxymikanolide, a sesquiterpene lactone, showed the strongest activity.[29]

Ethanolic extract and its hexane and ethyl acetate fractions of M. laevigata and M. glomerata were tested against oral pathogens. The hexane fraction from both plant extracts was the most effective in inhibiting the growth of the tested bacterial strains (minimum inhibitory concentration [MIC] values between 12.5 g/ml and 400 g/ml and minimum bactericidal concentration values between 25 and 400 g/ml, respectively), indicating that the biologically active substances have low polarity and are present mostly in the hexane fraction of both Mikania species, which showed remarkable inhibitory activities against Streptococcus mutans.[30]

The hexane extract of M. glomerata was tested against the multiresistant strain of S. aureus PI57, whereby it showed significant inhibition zone.[31] From the same species, an extract with high content in kaurenoic acid was tested in vitro against several cariogenic bacteria achieving to inhibit the growth of microorganisms responsible for dental caries at relatively low MIC values.[32] For the same species, the antibacterial activity against S. aureus of a hydromethanolic extract was evaluated, showing positive result and also a synergic effect with some antibacterial drugs already widely in use.[33]

The effect of a tincture from M. glomerata on Streptococcus mutatis and Streptococcus oralis, an oral bacterium that cause tooth losses, was evaluated which manifested bacteriostatic and bactericide effects.[34] Another article reports the potential activity of an extract rich in ent-kaurenoic acid obtained from M. glomerata against bacteria that can cause endodontic infections. It states that this extract and its major constituent ent-kaurenoic acid show in vitro antibacterial activity, the latter being a potential biofilm inhibitory agent.[35] The effect of extracts and formulations (antiseptic solutions and syrups) containing M. glomerata, with or without propolis, on bacterial growth was evaluated on S. mutans ATCC 25175 by agar diffusion method and both, extracts and formulations containing this species, were active against the bacteria.[36]

Diterpenes isolated from leaves of Mikania hirsutissima were evaluated for antibacterial activity on microorganisms responsible for bovine mastitis, whereby they presented satisfactory MIC (1.56–6.25 μg/ml) against S. aureus (ATCC and clinical isolate), Staphylococcus epidermidis, Streptococcus agalactiae, and Streptococcus dysgalactiae, thus this derivative can be used to control Gram-positive bacteria related to bovine mastitis.[37]

The antibacterial activity of an ethanolic extract of Mikania cordata was tested against Salmonella typhi, Shigella sonnei, Proteus spp., Pseudomonas aeruginosa, Enterococci, Streptococcus pyogenes, Shigella flexneri, Shigella dysenteriae, S epidermidis, and S. aureus, both Gram-positive and Gram-negative bacteria, and showed moderate results.[38]

Effect on smooth muscle

The bronchodilator activity of M. glomerata Sprengel derivatives (aqueous and hydroethanolic extracts and dichloromethane fraction) was tested on human bronchi and guinea-pig trachea, both presented relaxation activity and inhibited the histamine contractions, indicating that M. glomerata products are useful to treat respiratory diseases where bronchoconstriction is present. In the same experiment, the dichloromethane fraction showed also a light vasodilator effect on the isolated mesenteric vascular bed and on isolated rat aorta.[39] The antispasmodic effects of M. micrantha Kunth and M. cordifolia (L. F.) Willd aqueous extract were verified on isolated rat intestine, the observed antispasmodic effect could be associated to a noncompetitive Ca2+-influx.[40]

Antifungal activity

The antifungal activity of deoxymikanolide, scandenolide, dihydroscandenolide, mikanolide, dihydromikanolide, and m-methoxybenzoic acid, substances isolated from M. micrantha chloroform extract, was evaluated, and all the isolated compounds were effective against the tested strains, showing deoxymikanolide, a sesquiterpene lactone, with the strongest activity.[29]

The essential oil and extracts (chloroform, ethyl acetate, and methanol) of M. scandens were tested for antifungal activity, which was assayed using disc diffusion technique and determining MIC. The samples showed an important potential antifungal activity as mycelial growth inhibitor, against the tested phytopathogenic fungi.[41] The essential oil of M. glomerata exhibits anti-candida action verified by MIC test, which may be associated to the presence of terpene constituents in the oil.[42]

Antiparasitic activity

Some terpenes present antiparasitic activity, like the kaurenoic acid, isolated from an ethanolic extract from Mikania obtusata leaves and characterized as a trypanocidal component of the extract.[43] The diterpene ent-pimara-8 (14),15-dien-19-oic acid and three thymol derivatives, 10-acetoxy-8,9-dehydro-6-methoxythymol butyrate, 10-acetoxy-8,9-epoxy-6-methoxythymol isobutyrate, and acetylschizoginol, from M. decora exhibit in vitro anti-leishmanial and trypanocidal activities against Leishmania amazonensis, axenic amastigotes, and Trypanosoma cruzi, trypomastigotes,[24] and the diterpene ent-9alfa-hydroxy-15b-E-cinnamoyloxy-16-kauren-19-oic acid obtained from Mikania stipulacea and the sesquiterpene lactone-8beta-hydroxyzaluzanin-D isolated from Mikania hoehnei were tested on T. cruzi Y strain, both compounds being active.[44]

Mutagenic effect

The capacity to induce DNA damages and the mutagenic effects of the infusion from M. glomerata (IM) were evaluated in vitro, on hepatoma tissue culture cells, with comet assay and micronucleus test. The extracts tested at different doses were prepared differently as infusion and macerate in 80% ethanol from M. glomerata. In the comet assay, all extracts demonstrated genotoxic effects, but in the micronucleus test, except at 40 μL IM/mL culture medium, all treatments were not mutagenic, and these effects did not show direct relation to the coumarin quantity present in infusion and macerate. The results demonstrated DNA damages at the highest concentrations of alcoholic macerate (10 and 20 μL/mL) and infusion of M. glomerata (20 and 40 μL/mL), therefore, both dose and preparation form shall be used with caution.[45] However, another study, using a dichloromethane fraction obtained from the hydroethanolic extract of M. glomerata leaves when tested on plasmid DNA, did not damage the DNA.[39]

Monoamine oxidase inhibitory activity

The inhibitory activity on monoamine oxidase (MAO) of M. glomerata preparations was evaluated using a mitochondrial suspension where the hexane and dichloromethane extracts were active on the MAO-B isoform, without effect on MAO-A isoform, while the methanolic extract presented inhibitory activity on both isoforms (MAO-A and MAO-B), thus showing no selectivity.[31]

Antiviral activity

The investigation of compounds isolated from M. micrantha revealed that some sesquiterpene lactones exhibited antiviral action against influenza virus, which brings an important perspective for new therapies against respiratory virus.[46]

In vivo studies

Antiulcer activity

The antiulcer activity of the alkaloid fraction obtained from an ethanolic extract of the leaves of M. cordata was evaluated using a sodium diclofenac-induced gastric ulcer model and the results of the study revealed that this fraction from M. cordata shows antiulcer effects.[47],[48] Another preparation, a methanolic extract from root of M. cordata (Burm., B. L. Robinson), was investigated for a possible ulcer-protective activity on male Sprague-Dawley rats, and it was found that M. cordata root extract possesses antiulcer activity and that the observed activity may be due to the modulation of defensive factors through an improvement of gastric cytoprotection.[49]

The hydroethanolic extract of M. laevigata and coumarin obtained from it were tested aiming to evaluate their activity in gastric ulcer using different animal models, where the crude hydroalcoholic extract reduced the ulcerative lesion index induced by indomethacin, ethanol, stress, and reserpine in rats and, in the pyloric ligation model, a decrease of 53% was observed, suggesting that the pharmacological mechanism is related to an antisecretory activity that may be mediated by the parasympathetic system.[50]

Antibacterial activity

The antibacterial and cytotoxic properties of ethanolic extract from leaves of M. cordata were tested against four Gram-positive and six Gram-negative bacteria at different concentrations using the disc diffusion method, where the extract showed moderate antibacterial action mainly against S. flexneri. Comparatively, Gram-positive bacteria demonstrated more susceptibility to the extract than the Gram-negative bacteria, and the cytotoxic property of the sample was verified using Brine shrimp lethality bioassay where it did not show noticeable toxicity.[51]

Hepatoprotective effect

The hepatoprotective effect of M. scandens was tested in animal model using rats, in which the injury was produced by ethyl alcohol and drugs such as paracetamol and sodium diclofenac, showing that ethanolic extract and fractions have a promising hepatoprotective effect.[52],[53]

The effect of a methanolic fraction of a petroleum ether extract obtained from roots of M. cordata (Burm., B. L. Robinson) was investigated on a possible ulcer protective activity in male Sprague-Dawley rats. The evaluation could determine its effects on Phases 1 and 2 of the hepatic drug-detoxifying enzyme system in rats. It was found to have very little or, actually, no effect on hepatic microsomal cytochrome P-450 and cytochrome b contents, as well as NADPH cytochrome C reductase activity. It caused a remarkable induction of uridine diphosphate-glucuronyltransferase (UDP-GT) activities of liver microsomes. The extract also significantly increased the activity of microsomal uridine diphosphoglucose dehydrogenase and reduced nicotinamide adenine dinucleotide phosphate: quinine reductase and cytosolic glutathione (GSH) S-transferases, with a concomitant elevation in the contents of reduced GSH. These effects were found to be dose dependent and maintained during 12-week treatment period, indicating that the intracellular contents of active intermediates of various xenobiotics including chemical carcinogens would be reduced by the specific enhancement of drug-detoxifying enzymes in the liver of rats treated with the plant extract.[49]

Effects on central nervous system

The ethanolic extract from leaves of M. scandens shows CNS depressant effects in mice.[18] Neuropharmacological assays using hydroethanolic extracts from aerial parts and roots of the same species showed, in experimental animal models, significant and dose-dependent central anti-nociceptive, locomotor depressant, muscle relaxant, and sedative-potentiating effects, demonstrating its depressant action on the CNS, indicating that both aerial parts and roots of M. scandens possess prominent depressant action on the CNS, as manifested in these neuropharmacological experiments on mice.[54],[55]

The methanolic extract of M. cordata root possesses significant CNS depressant action and also analgesic activity.[56] Similarly, the effects of ethanol extract of leaves of M. glomerata, standardized, were verified on the amino acids of the hippocampus, leading to sedative and anxiolytic actions, which may be mediated by GABAergic system, and was able to increase gamma- aminobutyric acid (GABA) levels and reduce glutamate and aspartate concentrations in mouse hippocampus, which can directly and/or indirectly assist in their anxiolytic effect.[57]

Antiprotozoal activity

The antiprotozoal effect of aqueous and organic, dichloromethane/methanol (1:1), extracts from four Mikania species was tested against T. cruzi and Leishmania braziliensis. The organic extracts from M. micrantha, M. periplocifolia, M. parodii, and M. cordifolia showed significant in vitro antiprotozoal activity against T. cruzi, epimastigotes, and L. braziliensis, promastigotes. The M. micrantha organic extract was the most active against the two protozoans. All aqueous extracts showed moderate-to-low activity against T. cruzi and L. braziliensis.[58]

Anti-ophidian activity

In 2005, Maiorano et al.[59] evaluated aqueous extracts prepared from fresh dried roots, stems, and leaves of M. glomerata, where the extracts efficiently neutralized different toxic, pharmacological, and enzymatic effects induced by Bothrops and Crotalus snake venoms. Phospholipase A2 activity and the edema induced by Crotalus durissus terrificus venom were also inhibited. The hemorrhagic activity of Bothrops venoms and the clotting activity of C. durissus terrificus, Bothrops jararacussu, and Bothrops neuwiedi venoms were totally inhibited.[59] In addition, the dichloromethane fraction of the hydroalcoholic extract from the same species had a significant reduction of the edema induced by subplantar injections of Bothrops jararaca venom in mice.[39]

In another study, the hydroethanolic leaf extract from M. glomerata showed significant activity on Wistar rats treated with B. jararaca snake venom depicted as a significant reduction of the inflammation cells migration, a marked decrease in edema and also a significant reduction in the intensity of the hemorrhagic effects.[60]

For the same species, M. glomerata, the levels of pro-inflammatory and anti-inflammatory cytokines were evaluated, as well as the effect of the conventional treatment against snakebite in comparison to the effect of this treatment was complemented with extract from M. glomerata in experimental intoxication by Bothropoides jararaca. The results showed that botulinum toxin poisoning mainly stimulated the production of serum interleukin (IL)-6 and tumor necrosis factor-alpha, IL-1β, and IL-6 in the paw homogenate of experimentally intoxicated animals. The complementary treatment with the extract from M. glomerata had a major influence on the production of IL-6, IL-10, and IFN-γ in the serum and IL-6, IL-1β, and IFN-γ in the homogenate.[61]

Antidiarrheal activity

The investigation of the antidiarrheal activity of the aqueous extract from leaves of M. glomerata showed a decrease in the propulsive movements of the intestinal contents in mice. Oral administration produced an inhibition of gastrointestinal transit as effective as that produced by loperamide. These findings suggest that the aqueous extract of the leaves of M. glomerata might elicit an antidiarrheal effect by inhibiting intestinal motility.[62]

The antidiarrheal activity of the ethanolic extract of the leaves of M. cordata was evaluated in mice and it significantly reduced the number of bowel movements, when compared to a control group.[63]

Cytotoxicity and genotoxic activities

The n-butanolic extract of M. micrantha-containing flavonoids was selected for anticancer activity against Ehrlich Ascites Carcinoma (EAC) cell line cell line in Swiss albino mice where the extract expressed dose-dependent anticancer activity attributed to the presence of polyphenolic groups.[64] Moreover, the potential biological activity of the hydroethanolic extract of M. laevigata on the genotoxicity induced by alkylating agents (methyl methanesulfonate and cyclophosphamide), using the comet assay, was verified, and the results showed that the treatment with M. laevigata extract, previously compared to methyl methanesulfonate and cyclophosphamide administration, reduced DNA damage in mice.[65]

Antinociceptive and anti-inflammatory activities

Ethanolic extract from dried leaves of M. cordata was tested for its possible antinociceptive, cytotoxic, and antibacterial activities in animal models, where the extract produced significant writhing inhibition in acetic acid model in mice at the oral doses of 125 and 250 mg/kg body weight (P< 0.001), comparable to the standard drug, sodium diclofenac, at the dose of 25 mg/kg of body weight. The crude extract produced moderate cytotoxic activity against brine shrimp Artemia salina (LC50 =90 and LC90 =166 μg/ml).[38]

The crude extract of M. cordata (crude petrol: Et2O:MeOH 1:1:1) and an isolated sesquiterpene, deoxymikanolide, significantly inhibited acetic acid-induced writhing in mice, suggesting an antinociceptive activity of M. cordata extract.[66]

The aqueous extract of Mikania lindleyana exhibited anti-inflammatory and antinociceptive activities in different animal models.[67] In addition, the methanolic extract of M. lindleyana presented anti-inflammatory and antinociceptive activities that may be related to opioid mechanism.[68]

Carrageenan-induced inflammation was significantly antagonized by M. scandens hydroethanolic leaf extract, showing 50% inhibition at a dose of 1000 mg/kg.[15] The study of the antinociceptive activity of a hydroethanolic extract from flowers of M. scandens showed significant dose-dependent anti-nociceptive, locomotion skeletal muscle relaxation, and sedative-potentiating effects (P< 0.001), demonstrating its depressant action on the murine CNS. It allows to conclude that the flower of M. scandens possesses a prominent antinociceptive property along with marked hasty action on the CNS of mice.[16]

Analgesic activity of methanolic extract of M. scandens was investigated using the tail flick technique in mice, where it presented a distinct analgesic property.[69]

The anti-inflammatory activity of the sesquiterpene lactones from M. micrantha was tested in ear model of edema induced by Acetate 12-O-tetradecanoylphorbol (TPA), where they exhibited moderate activity.[22]

Ethanolic extract from leaves of M. micrantha was used to evaluate the central and peripheral antinociceptive activity where it was observed that this extract produced significant analgesic activity, both centrally and peripherally; in addition, the acute oral toxicity test showed no sign of toxicity or mortality.[70]

The hydroethanolic extract and isolated substances (coumarin and o-coumaric acid) of M. laevigata leaves were tested in a model of allergic pneumonitis using mice, where it was possible to observe reduction, after the treatment period, of the influx of inflammatory cells into the site of the lesion when compared to the control, mainly eosinophils, which demonstrates an important activity of the extract, and its constituents, in allergic inflammation.[11] Dichloromethane fractions (MG) obtained from the ethanolic extract of M. glomerata were evaluated for possible antiallergenic effect and anti-inflammatory activity on models of ovalbumin-induced allergic pleurisy and in models of local inflammation induced by biogenic amines, carrageenan, and platelet-activating factor (PAF). Plasma exudation as well as neutrophil and eosinophil infiltration evoked by the pleural injection of the antigen was significantly reduced by the fraction. Likewise, PAF-induced pleural neutrophil migration was inhibited by the treatment with a dichloromethane fraction, but pretreatment of the animals with dichloromethane fraction failed to modify the pleurisy induced by histamine, serotonin, or carrageenan. This suggests that MG1 is effective in inhibiting immunologic inflammation but does not affect acute inflammatory response caused by other agents.[10]

By evaluating the effect of pretreatment with extracts of M. glomerata and M. laevigata in inflammation and oxidative stress in the lungs of rats exposed only to intratracheal inhalation of coal dust, it was observed that after 15 days of treatment, a reduction in cell count can be observed, suggesting that both extracts play a role in the prevention of oxidative injuries in lung, caused by exposure to coal dust.[12]

The anti-inflammatory activity of the hydroethanolic extract of M. laevigata was assayed by interference in vascular permeability and leukocyte function in peritonitis induced by carrageenan in mice. The extract decreases the vascular permeability and also the leukocyte rolling and adhesion into the inflamed tissues by a mechanism dependent on nitric oxide; this may occur by inhibition of pro-inflammatory cytokine production at the inflammatory site.[14]

Anti-inflammatory activity

Anti-inflammatory potential of leaf and stem decoctions of M. laevigata and Mikania involucrata was evaluated using paw edema and pleurisy models induced by carrageenan, in which the decoctions of the leaves of both species were more effective than those of stems in reducing the inflammatory compound in the model of paw edema. Moreover, by the pleurisy assay, decoctions of M. laevigata and M. involucrata leaves inhibited the migration of leukocytes to the pleural exudate.[13]


The in vivo toxicity evaluation of M. glomerata-standardized ethanolic extract was performed in mice that received oral severe doses of the extract for 30 consecutive days, leading to estimate LD50 at around 3000 mg/kg. A macroscopic morphological analysis of the main organs and the acute toxicity evaluation, performed using mouse biochemical and hematological parameters, allowed to classify the extract as nonnoxious, as it did not produce any morphological alterations in the main organs and in the biochemical and hematological parameters of the mice.[71]

A study that aimed to analyze the toxic effects of dried aqueous extract from M. glomerata during the gestational period of hypertensive rats allowed to observe that the extract from this species did not show the possibility of teratogenicity; it also showed no influence on vasoconstriction of hypertensive rats at the tested concentrations. The histological analysis leads to conclude that no significant alterations among the analyzed groups can be observed.[72]

The hydroalcoholic extract from M. glomerata produced no genotoxic damage in mice, but significantly increased the frequency of micronucleated polychromatic erythrocytes (MNPCEs) induced by doxorubicin, indicating a drug–drug interaction (potentiating the doxorubicin-induced mutagenicity). This rise was not accompanied by lipid peroxidation or antioxidant level reduction, as measured by malondialdehyde and the antioxidants GSH and Vitamin E. Despite the presence of coumarin (a known antioxidant), hydroalcoholic extract may exert adverse effects probably in association with mutagenic compounds, although this effect on DNA damage did not involve oxidative stress,[73] but in the analysis of the genotoxicity and cytotoxicity of the extract rich in kaurenoic acid from M. glomerata using the assay of frequency of MNPCEs and PCE/normochromatic erythrocytes ratio, it was not possible to observe any genotoxic or cytotoxic changes in mice.[32]

In the spermatogenic cycle of adult male rats, the hydroethanolic extract of M. glomerata did not significantly alter body and organ weights nor did interfere on gamete production, serum testosterone level, or food intake.[74] Moreover, the administration of hydroethanolic extract of M. glomerata did not show any impairment of fertility and no significant difference in the analyzed parameters, suggesting an absence of mutagenic effect on male Wistar rats.[75] The effect of the hydroalcoholic extract, prepared from aerial parts of M. glomerata, was also evaluated regarding the reproductive system of rats submitted to chronic treatment, in which no significant alteration of the analyzed variables was observed and the treatment did not affect the food consumption. These data suggest that the hydroalcoholic extract of M. glomerata shows no toxic effect and do not interfere on the fertility of Wistar rats, undergoing long-term treatment.[76]

Acute toxicity of the aqueous, petroleum ether, methanol, and chloroform extracts from M. scandens was evaluated by oral administration to different groups of mice at the doses of 1000, 2000, and 3000 mg/kg body weight. After administration of the extracts, animals were observed continuously for the first 3 h and continued at regular intervals for 24 h in order to detect any alteration in their behavior, but none was killed by the dose. Hence, it is proven that the drug has very low toxicity, at these doses.[69]

In the acute toxicity study of the extract from leaves of M. cordata where only female mice were treated with 5, 50, 300, and 2000 mg extract/kg body weight, respectively, the mice were monitored for 24 h after administration to determine any behavioral, neurological, and autonomic occurrence and, after 14 days, to verify mortality. During the observation period, neither behavioral change symptoms nor any mortality was recorded, so the extracts were considered safe.[63]

The n-butanolic extract from M. micrantha containing flavonoids was tested for anticancer activity against EAC cell line in Swiss albino mice and also to check the acute toxicity, where the extract's dose-dependent anticancer activity could be attributed to the presence of polyphenolic groups. Furthermore, the extract did not show any toxic reactions and mortality up to a dose of 2000 mg/kg.[64]

Clinical studies

Among few clinical studies about Mikania species, most of them report studies performed using syrups and mainly ointments containing derivatives of this species. One of these shows the clinical safety in using an herbal formulation combining M. glomerata and other species to treat respiratory diseases used in 26 healthy adult volunteers of both sexes during 28 uninterrupted days where, after the necessary analysis, no toxicity was observed.[77]

In addition, the clinical safety of a syrup containing “guaco” tincture (M. glomerata), in association with other species (Grindelia robusta, Copaifera officinalis, Myroxylon toluifera, and Nasturtium officinale), the main indication of which is to treat respiratory affections, was evaluated and it was well tolerated in an uncontrolled clinical trial on 24 volunteers of both sexes, who received 15 mL of the syrup, four times daily for 21 uninterrupted days without serious adverse events.[78]

A study performed to evaluate the safety and the genotoxic potential of “Melagrião,” an herbal medicine composed by six plant extracts among them, M. glomerata, was carried out on adult volunteers for 28 days, evaluating the clinical toxicology and genotoxicity, as result no evidences of toxicity were detected.[79]

Regarding the kinetic profile of the main metabolites of guaco syrup, the evaluation was performed in healthy humans who received 60 mL of syrup containing 1500 mg of coumarin. After administration, the kinetic profile in the blood was evaluated and the result showed that o-coumaric acid is one of the main bioavailable metabolites of coumarin and not 7-hydroxycoumarin; therefore, the hydrolysis of the lactone ring forming a carboxylated compound is one of the possible metabolic routes for coumarin in humans.[80]

A study conducted to determine the safety and efficacy of an ointment containing methanolic extract of M. cordata showed the safety of the formulation and that it is pharmacologically comparable to mupirocin and therefore could be used for the treatment of superficial lesions.[81]

   Conclusion Top

Genus Mikania is distributed throughout Brazil predominating in the southern and southeastern regions. It comprises species of pharmaceutical and phytochemical interests too. This systematic review shows that there are many popular alleged uses and a substantial number of articles dealing with different pharmacological activities for different species of the genus, either in vitro or in vivo[Table 2]. However, the number of clinical and pharmacokinetic studies reported is still low, even though M. glomerata and M. laevigata are already inserted in the formulary of phytomedicines of the Brazilian Pharmacopoeia.
Table 2: In vitro and in vivo studies with Mikania genus

Click here to view

The most studied species so far are M. glomerata, M. laevigata, M. scandens, and M. micrantha, the first two being widely used in Brazil to treat respiratory disorders and are present in various pharmaceutical formulations in the local retail market.

Among the pharmacological activities, anti-inflammatory, analgesic, antibacterial, and CNS were the most referred, which are directly related to the alleged popular use of this genus species. In addition, a great number of toxicological tests on animals have also been carried out. This systematic review intends to gather the pharmacological knowledge published about Mikania species and thus contribute to give scientific base for a safe use of derivatives from the species here cited.


We would like to thank CAPEs for the scholarship granted and the UFPA for the facilities.

Financial support and sponsorship

ASBS thanks CAPES - Coordination for the Improvement of the Academic Personnel for the grant received during her doctorate.

Conflicts of interest

There are no conflicts of interest.

   References Top

WHO. WHO traditional medicine strategy 2014-2023. Organ Mund Salud 2013;72:7-8. Available from: http://www.apps.who.int/medicinedocs/documents/s21201es/s21201es.pdf.[Last accessed on 2018 Mar 13].  Back to cited text no. 1
Harvey A. Strategies for discovering drugs from previously unexplored natural products. Drug Discov Today 2000;5:294-300.  Back to cited text no. 2
Sponchiado G, Adam ML, Silva CD, Soley BS, de Mello-Sampayo C, Cabrini DA, et al. Quantitative genotoxicity assays for analysis of medicinal plants: A systematic review. J Ethnopharmacol 2016;178:289-96.  Back to cited text no. 3
Dutra RC, Campos MM, Santos AR, Calixto JB. Medicinal plants in Brazil: Pharmacological studies, drug discovery, challenges and perspectives. Pharmacol Res 2016;112:4-29.  Back to cited text no. 4
ANVISA. Formulário de Fitoterápicos Farmacopeia Brasileira. Brazilian Pharmacopea. 1st. edition 2011. p. 1-126.  Back to cited text no. 5
Di Stasi LC, Oliveira GP, Carvalhaes MA, Queiroz M Jr. Tien OS, Kakinami SH, et al. Medicinal plants popularly used in the Brazilian tropical Atlantic forest. Fitoterapia 2002;73:69-91.  Back to cited text no. 6
Silva Junior AA, Ritter MR, Zambonim FM, Deschamps FC, Tcacenco FA, Bertoldi FC. New ecotype of Mikania glomerata Spreng (Asteraceae) rich in essential oil in Southern Brazil. Rev Fitos 2015;9:19-28. Available from: http://www.gnresearch.org/doi/10.5935/2446-4775.20150002. [Last accessed on 2018 Mar 13].  Back to cited text no. 7
Ritter MR, Waechter JL. Biogeography of the genus Mikania Willd. (Asteraceae) in Rio Grande of South, Brazil. Acta Bot Bras 2004;18:643-52.  Back to cited text no. 8
Rufatto LC, Gower A, Schwambach J, Moura S. Genus Mikania: Chemical composition and phytotherapeutical activity. Brazilian J Pharmacogn 2012;22:1384–403.  Back to cited text no. 9
Fierro IM, da Silva AC, Lopes Cda S, de Moura RS, Barja-Fidalgo C. Studies on the anti-allergic activity of Mikania glomerata. J Ethnopharmacol 1999;66:19-24.  Back to cited text no. 10
dos Santos SC, Krueger CL, Steil AA, Kreuger MR, Biavatti MW, Wisniewski Junior A, et al. LC characterisation of guaco medicinal extracts, Mikania laevigata and M. Glomerata, and their effects on allergic pneumonitis. Planta Med 2006;72:679-84.  Back to cited text no. 11
Freitas TP, Silveira PC, Rocha LG, Rezin GT, Rocha J, Citadini-Zanette V, et al. Effects of Mikania glomerata spreng. And Mikania laevigata schultz bip. Ex baker (Asteraceae) extracts on pulmonary inflammation and oxidative stress caused by acute coal dust exposure. J Med Food 2008;11:761-6.  Back to cited text no. 12
Suyenaga ES, Reche E, Farias FM, Schapoval EE, Chaves CG, Henriques AT, et al. Antiinflammatory investigation of some species of Mikania. Phytother Res 2002;16:519-23.  Back to cited text no. 13
Alves CF, Alves VB, de Assis IP, Clemente-Napimoga JT, Uber-Bucek E, Dal-Secco D, et al. Anti-inflammatory activity and possible mechanism of extract from Mikania laevigata in carrageenan-induced peritonitis. J Pharm Pharmacol 2009;61:1097-104.  Back to cited text no. 14
Banerjee S, Chanda A, Adhikari A, Das A, Biswas S. Evaluation of phytochemical screening and anti inflammatory activity of leaves and stem of Mikania scandens (L.) Wild. Ann Med Health Sci Res 2014;4:532-6.  Back to cited text no. 15
[PUBMED]  [Full text]  
Bhattacharya S, Chandra S, Dey P. Antinociceptive activity of Mikania scandens flower in albino mice: Involvement of CNS depressant role. Orient Pharm Exp Med 2013;13:199-204.  Back to cited text no. 16
Hasan SM, Jamila M, Majumder MM, Akter R, Hossain M, Mazumder EH, et al. Analgesic and antioxidant activity of the hydromethanolic extract of Mikania scandens (L.) Willd. leaves. Am J Pharmacol Toxicol 2009;4:1-7.  Back to cited text no. 17
Pal D, Mazumder UK. Isolation of compound and CNS depressant activities of Mikania scandens Willd with special emphasis to brain biogenic amines in mice. Indian J Exp Biol 2014;52:1186-94.  Back to cited text no. 18
Duarte MC, Leme EE, Delarmelina C, Soares AA, Figueira GM, Sartoratto A. Activity of essential oils from Brazilian medicinal plants on Escherichia coli. J Ethnopharmacol 2007;111:197-201.  Back to cited text no. 19
Gasparetto JC, Campos FR, Budel JM, Pontarolo R. Mikania glomerata Spreng. Agronomic, genetic, morphoanatomical, chemical, pharmacological and toxicological studies and use in phytotherapy programs in Brazil. Brazilian J Pharmacogn 2010;20:627-40.  Back to cited text no. 20
Rufatto LC, Finimundy TC, Roesch-Ely M, Moura S. Mikania laevigata: Chemical characterization and selective cytotoxic activity of extracts on tumor cell lines. Phytomedicine 2013;20:883-9.  Back to cited text no. 21
Ríos EV, León A, Chávez MI, Torres Y, Ramírez-Apan MT, Toscano RA, et al. Sesquiterpene lactones from Mikania micrantha and Mikania cordifolia and their cytotoxic and anti-inflammatory evaluation. Fitoterapia 2014;94:155-63.  Back to cited text no. 22
Dou X, Zhang Y, Sun N, Wu Y, Li L. The anti-tumor activity of Mikania micrantha aqueous extract in vitro and in vivo. Cytotechnology 2014;66:107-17.  Back to cited text no. 23
Aponte JC, Jin Z, Vaisberg AJ, Castillo D, Málaga E, Lewis WH, et al. Cytotoxic and anti-infective phenolic compounds isolated from Mikania decora and Cremastosperma microcarpum. Planta Med 2011;77:1597-9.  Back to cited text no. 24
Education AP. Comparative in vitro evaluation of anti-inflammatory effects of aerial parts and roots from. J Adv Pharm Educ Res 2011;1:271-7.  Back to cited text no. 25
Peluso G, De Feo V, De Simone F, Bresciano E, Vuotto ML. Studies on the inhibitory effects of caffeoylquinic acids on monocyte migration and superoxide ion production. J Nat Prod 1995;58:639-46.  Back to cited text no. 26
Ghosh A, Das BK, Roy A, Mandal B, Chandra G. Antibacterial activity of some medicinal plant extracts. J Nat Med 2008;62:259-62.  Back to cited text no. 27
Facey PC, Peart PC, Porter RB. The antibacterial activities of mikanolide and its derivatives. West Indian Med J 2010;59:249-52.  Back to cited text no. 28
Li Y, Li J, Li Y, Wang XX, Cao AC. Antimicrobial constituents of the leaves of Mikania micrantha H. B. K. PLoS One 2013;8:e76725.  Back to cited text no. 29
Yatsuda R, Rosalen PL, Cury JA, Murata RM, Rehder VL, Melo LV, et al. Effects of Mikania genus plants on growth and cell adherence of mutans streptococci. J Ethnopharmacol 2005;97:183-9.  Back to cited text no. 30
do Amaral RR, Arcenio Neto F, Carvalho ES, Teixeira LA, De Araújo GL, Sharapin N, et al. Evaluation of MAOi and antibacterial activity of extracts of Mikania glomerata Sprengel. Rev Bras Farmacogn 2003;13:24-7. Available from: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2003000300010&lng=pt&nrm=iso&tlng=pt. [Last accessed on 2018 Mar 13].  Back to cited text no. 31
Moreira MR, Souza AB, Soares S, Bianchi TC, de Souza Eugênio D, Lemes DC, et al. Ent-kaurenoic acid-rich extract from Mikania glomerata: In vitro activity against bacteria responsible for dental caries. Fitoterapia 2016;112:211-6.  Back to cited text no. 32
Betoni JE, Mantovani RP, Barbosa LN, Di Stasi LC, Fernandes Junior A. Synergism between plant extract and antimicrobial drugs used on Staphylococcus aureus diseases. Mem Inst Oswaldo Cruz 2006;101:387-90.  Back to cited text no. 33
Pinheiro MA, Brito DB, Almeida LF, Cavalcanti YW, Padilha WW. Antimicrobial effect of tinctures of natural products on dental caries bacteria. Rev Bras Promoç Saúde 2012;25:197-201. Available from: http://www.redalyc.org/articulo.oa?id=40823359010. [Last accessed on 2018 Mar 13].  Back to cited text no. 34
Moreti DLC, Leandro LF, da Silva Moraes T, Moreira MR, Sola Veneziani RC, Ambrosio SR, et al. Mikania glomerata sprengel extract and its major compound ent-kaurenoic acid display activity against bacteria present in endodontic infections. Anaerobe 2017;47:201-8.  Back to cited text no. 35
Souza DH, Yamamoto CH, de Pinho JR, Alves MS, da Araújo LA, Sousa OV. Antibacterial activity against Streptococcus mutans and stability of natural products containing extract of Mikania glomerata Sprengel. Hum Rev 2006;32:11-4.  Back to cited text no. 36
Fonseca AP, Estrela FT, Moraes TS, Carneiro LJ, Bastos JK, dos Santos RA, et al. In vitro antimicrobial activity of plant-derived diterpenes against bovine mastitis bacteria. Molecules 2013;18:7865-72.  Back to cited text no. 37
Al Nayeem A, Khatun A, Rahman S, Rahman M. Evaluation of phytochemical and pharmacological properties of Mikania cordata (Asteraceae) leaves. Pharmacogn Phytother 2011;3:118-23. Available from: http://www.academicjournals.org/JPP/abstracts/abstracts/abstracts2011/Sept/AlNayeemetal.htm. [Last accessed on 2018 Mar 13].  Back to cited text no. 38
Soares de Moura R, Costa SS, Jansen JM, Silva CA, Lopes CS, Bernardo-Filho M, et al. Bronchodilator activity of Mikania glomerata sprengel on human bronchi and guinea-pig trachea. J Pharm Pharmacol 2002;54:249-56.  Back to cited text no. 39
Colares M, Muguerza A, Rosella MA, Consolini AE. Antispasmodic effects of Mikania micrantha Kunth and dual gastrointestinal effect of Mikania cordifolia (L.F.) Willd (Asteraceae) on isolated rat thin intestine. Pharmacologyonline 2013;2:1-11.  Back to cited text no. 40
Siddiqui SA, Islam R, Islam R, Jamal AH, Parvin T, Rahman A. Chemical composition and antifungal properties of the essential oil and various extracts of Mikania scandens (L.) Willd. Arab J Chem 2017;10:S2170-4. Available from: http://www.dx.doi.org/10.1016/j.arabjc. 2013.07.050. [Last accessed on 2018 Mar 13].  Back to cited text no. 41
Duarte MC, Figueira GM, Sartoratto A, Rehder VL, Delarmelina C. Anti-candida activity of Brazilian medicinal plants. J Ethnopharmacol 2005;97:305-11.  Back to cited text no. 42
Alves TM, Chaves PP, Santos LM, Nagem TJ, Murta SM, Ceravolo IP, et al. A diterpene from Mikania obtusata active on Trypanosoma cruzi. Planta Med 1995;61:85-7.  Back to cited text no. 43
do Nascimento AM, Siqueira Chaves J, Albuquerque S, Rodrigues de Oliveira DC. Trypanocidal properties of Mikania stipulacea and Mikania hoehnei isolated terpenoids. Fitoterapia 2004;75:381-4.  Back to cited text no. 44
Costa Rde J, Diniz A, Mantovani MS, Jordão BQ. In vitro study of mutagenic potential of Bidens pilosa linné and Mikania glomerata sprengel using the comet and micronucleus assays. J Ethnopharmacol 2008;118:86-93.  Back to cited text no. 45
But PP, He ZD, Ma SC, Chan YM, Shaw PC, Ye WC, et al. Antiviral constituents against respiratory viruses from Mikania micrantha. J Nat Prod 2009;72:925-8.  Back to cited text no. 46
Mosaddik MA, Alam KM. The anti-ulcerogenic effect of an alkaloidal fraction from Mikania cordata on diclofenac sodium-induced gastrointestinal lesions in rats. J Pharm Pharmacol 2000;52:1157-62.  Back to cited text no. 47
Paul RK, Jabbar A, Rashid MA. Antiulcer activity of Mikania cordata. Fitoterapia 2000;71:701-3.  Back to cited text no. 48
States C, Reed E. Cancer letters. Cancer Lett 1996;108:233-7.  Back to cited text no. 49
Bighetti AE, Antônio MA, Kohn LK, Rehder VL, Foglio MA, Possenti A, et al. Antiulcerogenic activity of a crude hydroalcoholic extract and coumarin isolated from Mikania laevigata schultz bip. Phytomedicine 2005;12:72-7.  Back to cited text no. 50
Ali MS, Islam MS, Rahman MM, Islam MR, Sayeed MA, Islam MR, et al. Antibacterial and cytotoxic activity of ethanol extract of Mikania cordata (burm.f.) B.L. Robinson leaves. J Basic Clin Pharm 2011;2:103-7.  Back to cited text no. 51
Maity T, Ahmad A, Pahari N. Evaluation of hepatotherapeutic effects of Mikania scandens (L.) Willd. on alcohol induced hepatotoxicity in rats. Int J Pharm Pharm Sci 2012;4:490-4.  Back to cited text no. 52
Maity T, Ahmad A, Pahari N. Hepatoprotective activity of Mikania scandens (L.) Willd. against diclofenac sodium induced liver toxicity in rats. Acad Sci 2012;5:185-9.  Back to cited text no. 53
Bhattacharya S, Dey P, Chandra S, Chatterjee P. Neuropharmacological properties of Mikania scandens (L.) Willd. (Asteraceae). Adv Pharm Technol Res 2011;2:255. Available from: http://www.japtr.org/text.asp?2011/2/4/255/90883. [Last accessed on 2018 Mar 13].  Back to cited text no. 54
Dey P, Chandra S, Bhattacharya S. Neuropharmacological activities of Mikania scandens root. Glob J Pharmacol 2012;6:193-8.  Back to cited text no. 55
Bhattacharya S, Pal S, Chaudhuri AK. Neuropharmacological studies on Mikania cordata root extract. Planta Med 1988;54:483-7.  Back to cited text no. 56
Santana LC, Brito MR, Oliveira GL, Citó AM, Alves CQ, David JP, et al. Neurochemical Study. Evid Based Complement Alternat Med 2014;2014:1-11.  Back to cited text no. 57
Laurella LC, Frank FM, Sarquiz A, Alonso MR, Giberti G, Cavallaro L, et al. In vitro evaluation of antiprotozoal and antiviral activities of extracts from Argentinean mikania species. ScientificWorldJournal 2012;2012:121253.  Back to cited text no. 58
Maiorano VA, Marcussi S, Daher MA, Oliveira CZ, Couto LB, Gomes OA, et al. Antiophidian properties of the aqueous extract of Mikania glomerata. J Ethnopharmacol 2005;102:364-70.  Back to cited text no. 59
Mourão VB, Giraldi GM, Neves LM, Gaspi FO, Rodrigues RA, Alves AA, et al. Anti-hemorrhagic effect of hydro-alcoholic extract of the leaves of Mikania glomerata in lesions induced by Bothrops jararaca venom in rats. Acta Cir Bras 2014;29:30-7.  Back to cited text no. 60
Motta YP, Sakate M, Nogueira RM, Peraçoli MT, Sangiorgio F, Floriano RS, et al. Quantification of cytokines in the paw homogenate serum in the experimental poisoning with Bothropoides jararaca venom in Wistar rats treated with serum therapy and Mikania glomerata. Arq Bras Med Vet Zootec 2014;66:1413-8.  Back to cited text no. 61
Salgado HR, Roncari AF, Moreira RR. Antidiarrhoeal effects of Mikania glomerata Spreng. (Asteraceae) leaf extract in mice. Brazilian J Pharmacogn 2005;15:205-8.  Back to cited text no. 62
Nasrin F, Hakim ML. In vivo antidiarrheal study of ethanolic extracts of Mikania cordata and Litsea monopetala leaves. Bangladesh J Pharmacol 2015;10:562-5.  Back to cited text no. 63
Debaprotim D, Suvakanta D, Jashabir C. Evaluation of anticancer activity of Mikania micrantha Kunth (Asteraceae) against ehrlich ascites carcinoma in Swiss albino mice. Int J Pharm Res Allied Sci 2014;3:9-18.  Back to cited text no. 64
Medeiros Mazzorana D, Nicolau V, Moreira J, de Aguiar Amaral P, de Andrade VM. Influence of Mikania laevigata extract over the genotoxicity induced by alkylating agents. ISRN Toxicol 2013;2013:521432.  Back to cited text no. 65
Ahmed M, Rahman MT, Alimuzzaman M, Shilpi JA. Analgesic sesquiterpene dilactone from Mikania cordata. Fitoterapia 2001;72:919-21.  Back to cited text no. 66
Silva AS, Pinheiro BG, Figueiredo JG, et al. Antinociceptive and anti-inflammatory activities of the aqueous extract of Mikania lindleyana in rodents. IJPSR 2012;3:1637-46.  Back to cited text no. 67
Vanderlinde FA, Rocha FF, Malvar DC, Ferreira RT, Costa EA, Florentino IF, et al. Anti-inflammatory and opioid-like activities in methanol extract of Mikania lindleyana, Sucuriju. Braz J Pharmacogn 2011;22:150-6.  Back to cited text no. 68
Chakraverty R, Saha S. Extraction and phytochemical screening of Mikania scandens Linn. and evaluation of its methanolic extract for analgesic activity. IJPSR 2012;3:1430-2.  Back to cited text no. 69
Packia Lincy KM, Paulpriya VM. Pharma science monitor. Pharma Sci Monit 2013;4:3947-63.  Back to cited text no. 70
Santana LC, Brito MR, Sousa GF, Freitas RM. Study of physical-chemical properties and acute toxicity evaluation of the ethanolic extract of the leaves of Mikania glomerata Sprengel. Rev Bras Plantas Med 2014;16:670-8.  Back to cited text no. 71
Fulanetti FB, Camargo GG, Ferro MC, Randazzo-Moura P. Toxic effects of the administration of Mikania glomerata Sprengel during the gestational period of hypertensive rats. Open Vet J 2016;6:23-9.  Back to cited text no. 72
Barbosa LC, de Morais MD, de Paula CA, da Silva Ferreira MC, Jordao AA, Andrade E Silva ML, et al. Mikania glomerata sprengel (Asteraceae) influences the mutagenicity induced by doxorubicin without altering liver lipid peroxidation or antioxidant levels. J Toxicol Environ Health Part A Curr Issues 2012;75:1102-9.  Back to cited text no. 73
Sa RC, Leite MN, de Moura Reporedo M, Nóbrega de Almeida R. Evaluation of long-term exposure to Mikania glomerata (Sprengel) extract on male Wistar rats' reproductive organs, sperm production and testosterone level. Contraception 2003;67:327-31.  Back to cited text no. 74
de Sá RC, Leite MN, Peters VM, Guerra MD, de Almeida RN. Absence of mutagenic effect of Mikania glomerata hydroalcoholic extract on adult wistar rats in vivo. Braz Arch Biol Technol 2006;49:599-604.  Back to cited text no. 75
de Sá RC, Leite MN, de Almeida RN. Toxicological screening of Mikania glomerata Spreng., Asteraceae, extract in male Wistar rats reproductive system, sperm production and testosterone level after chronic treatment. Rev Bras Farmacogn 2010;20:718-28.  Back to cited text no. 76
Tavares JP, Martins IL, Vieira AS, Lima FA, Bezerra FA, Moraes MO, et al. Clinical toxicology study of a phytomedicine syrup composed of plants, honey and propolis. Rev Bras Farmacogn 2006;16:350-6. Available from: http://www.scielo.br/scielo.php?script=sci%7B_%7Darttext%7B&%7Dpid=S0102-695X2006000300012%7B&%7Dlang=pt. [Last accessed on 2018 Mar 13].  Back to cited text no. 77
Soares AK, Carmo GC, Quental DP, Nascimento DF, Bezerra FA, Moraes MO, et al. Clinical safety assessment of a herbal medicine containing Mikania glomerata, Grindelia robusta, Copaifera officinalis, Myroxylon toluifera, Nasturtium officinale, propolis and honey in healthy volunteers. Rev Bras Farmacogn 2006;16:447–54.  Back to cited text no. 78
Viana IOL. Clinical Toxicology Study and Therapeutic Efficacy of “Melagrião” phytomedicine. Fortaleza, CE, 2011, 206.  Back to cited text no. 79
Gasparetto JC, Peccinini RG, de Francisco TM, Cerqueira LB, Campos FR, Pontarolo R, et al. A kinetic study of the main guaco metabolites using syrup formulation and the identification of an alternative route of coumarin metabolism in humans. PLoS One 2015;10:e0118922.  Back to cited text no. 80
Herbert BE, Bagares LM, Galang RR, Garcines K, Go SS, Jalamana MA. Safety and Efficacy of herbal ointment formulated with methanolic extract of Mikania cordata as treatment for acute superficial injury. Res Rev J Pharmacogn Phytochem 2014;2:11-8.  Back to cited text no. 81


  [Figure 1]

  [Table 1], [Table 2]


    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

  In this article
    Materials and Me...
    Results and Disc...
    Article Figures
    Article Tables

 Article Access Statistics
    PDF Downloaded7    
    Comments [Add]    

Recommend this journal