|Year : 2018 | Volume
| Issue : 24 | Page : 208-213
A review study on pharmacological activities, chemical constituents, and traditional uses of Echium amoenum
Hossein Azizi1, Saloumeh Ghafari1, Roshanak Ghods2, Asie Shojaii3, Mahboubeh Salmanian1, Jafar Ghafarzadeh1
1 Department of Traditional Medicine, School of Persian Medicine, Tehran, Iran
2 Department of Traditional Medicine, School of Persian Medicine; Research Institute for Islamic and Complementary Medicine, Tehran, Iran
3 Research Institute for Islamic and Complementary Medicine; Department of Traditional Pharmacy, School of Persian Medicine, Iran University of Medical Sciences, Tehran, Iran
|Date of Web Publication||12-Oct-2018|
Department of Traditional Pharmacy, School of Persian Medicine; Iran and Research Institute for Islamic and Complementary Medicine, Iran University of Medical Sciences, Tehran
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Echium amoenum Fisch. and Mey. (Boraginaceae) is a plant which is used widely in Iranian folk medicine, especially for anxiety and depression. In this study, published scientific reports about the composition and pharmacological properties of this plant were reviewed. The electronic databases including Google Scholar, PubMed, ScienceDirect, Scopus, Web of Science, and Scientific Information Database were searched from 1970 to May 2016 and the data were summarized.Efficacy of E. amoenum (especially petals of E. amoenum) was studied in different in vitro, in vivo, and clinical evaluations. Furthermore, some chemical compounds such as rosmarinic acid, echimidine, and cyanogenic glycosides were isolated from E. amoenum petals. According to the results, E. amoenum showed various biological activities such as antimicrobial, antiviral, antioxidant, antidiabetic, analgesic, immunomodulatory, and anxiolytic effects. Clinical studies on E. amoenum showed effectiveness of this plant in depression and anxiety disorders. More clinical trials are recommended for evaluating different beneficial effects of this plant in human models and synthesis of new drugs from the active ingredients of this plant in the future.
Keywords: Biological activities, clinical, depression, chemical compound, Echium amoenum, in vivo, in vitro
|How to cite this article:|
Azizi H, Ghafari S, Ghods R, Shojaii A, Salmanian M, Ghafarzadeh J. A review study on pharmacological activities, chemical constituents, and traditional uses of Echium amoenum. Phcog Rev 2018;12:208-13
|How to cite this URL:|
Azizi H, Ghafari S, Ghods R, Shojaii A, Salmanian M, Ghafarzadeh J. A review study on pharmacological activities, chemical constituents, and traditional uses of Echium amoenum. Phcog Rev [serial online] 2018 [cited 2019 May 21];12:208-13. Available from: http://www.phcogrev.com/text.asp?2018/12/24/208/243194
| Introduction|| |
Echium amoenum Fisch. and Mey. is a medicinal plant that belongs to Boraginaceae family. This plant called “Gav Zaban” in Persian and “Lesan-al-sour” in Iranian traditional medicine. There are different species of Echium all around the world, but only four species of it are available in Iran.
E. amoenum is a biennial or perennial herb indigenous to the narrow zone of the northern part of Iran and Caucasus, where it grows at highlands at the altitude ranging from 60 to 2200 m. The petals of E. amoenum have been widely recommended for a variety of effects, such as sedative, anxiolytic, demulcent, anti-inflammatory, analgesic, antioxidant, and tranquilizing effects, and it is used as a decoction among the Iranian people in folk medicine.,,,,,, Despite the various studies which were conducted on E. amoenum, there is no comprehensive review about the chemical constituents and pharmacological activities of E. amoenum. Hence, in continuation of previous review studies on medicinal plants of Iranian traditional medicine, in this article, all published studies about E. amoenum were reviewed.,,
| Methods|| |
All published articles about the chemical constituents and pharmacological activities of E. amoenum were searched in different databases including Google Scholar, PubMed, ScienceDirect, Scopus, and Scientific Information Database from 1970 up to May 2016. The agricultural studies and investigations on tissue culture were excluded.
Echium amoenum in Iranian traditional medicine
E. amoenum was called “Lesan-al-sour” in Iranian traditional medicine. The temperament of it has been mentioned as warm and dry. The flowers of E. amoenum are used for the treatment of concern, cough, dyspnea, sore throat, grippe, and pneumonia in Iranian traditional texts. Some other effects such as efficacy in melancholy, obsession, fear, and icterus have been mentioned for this plant. Its flowers are known as exhilarant and tonic for heart, liver, spirit, and brain. It was also mentioned for the treatment of nephrolithiasis.,,
Evaluation of the composition of volatile oil from E. amoenum petals (yielded 0.05%) showed that it consists of 26 components. The major components, except aliphatic alkanes, which belong to sesquiterpenes, are ä-cadinene (24.25%), viridiflorol (4.9%), á-muurolene (4.52%), ledene (3.8%), á-calacorene (3.04%), ã-cadinene (2.9%).,
There are some compounds isolated from the extract of E. amoenum. Four pyrrolizidine alkaloids including echimidine, echimidine isomer, 7-angeloylretronecine, and 7-tigloylretronecine were isolated from dried petals of E. amoenum. The total alkaloid content of E. amoenum was 0.01%. The major phenolic component isolated from ethyl acetate extract of E. amoenum petals was rosmarinic acid, a potent antioxidant compound with antiviral and anti-inflammatory activities., Other compounds which have been reported of E. amoenum petals were cyanidin, delphinidin, anthocyanidins, gamma-linolenic acid, alpha-linolenic acid, δ-6-fatty acyl desaturase, δ-8-sphingolipid desaturase, and flavonoids ,,, [Figure 1].
|Figure 1: Structures of some chemical compounds isolated from Echium amoenum. (a) Rosmarinic acid; (b) echimidine; (c) echimidine isomer; (d) 7-Angeloyl retronecine; (e) 7-Tigloyl retronecine|
Click here to view
Evaluation of the nutritional composition of E. amoenum showed the presence of calcium, phosphorous, iron, and Vitamin C, in the concentration of 704, 356, 68, and 51 mg per 100 g of E. amoenum extract, respectively. Furthermore, the extract contained carotenoids and anthocyanin.
In vitro and in vivo studies
Antiviral activity of E. amoenum was evaluated against bacteriophage 3C by agar overlay method. The results showed that antiviral effect of the extract was heat resistant and was not eliminated by autoclaving at 110°C for 1 h. Although the activity of the freeze-dried extract was reduced during 90 days of storage at 4°C, the activity of working solution was diminished in a 1-week period at 4°C.
In another study, antiviral effect of E. amoenum extract was evaluated against HSV-1 by cytopathic effect inhibition assay. The extract showed significant antiviral activity and the most antiviral activity exhibited 1 h after virus inoculation. Antiviral activity of this plant was reduced after 2 and 3 h. The extract was inhibitor of virus replication at concentration higher than 400 μg/mL [Table 1].
Antibacterial activity of E. amoenum was evaluated against Staphylococcus aureus 8327 using agar well diffusion, disc diffusion, and minimum inhibitory concentration (MIC) methods. MIC of the extract was 6.2 μg/mL. The findings indicated concentration-dependent antibacterial activity of E. amoenum against S. aureus 8327 and the antibacterial activity was heat resistance.,, In contrast to this study, another study did not show significant activity of E. amoenum extract against these bacteria.
In a study by Bonjar, methanolic extract of E. amoenum was tested against Micrococcus luteus, Serratia marcescens, Klebsiella pneumonia, and Bordetella bronchiseptica by disc diffusion and MIC methods. The result showed that the extract was effective against B. bronchiseptica with MIC 15 mg/mL.
Another antimicrobial evaluation was assessed at various concentrations of seed oil of E. amoenum (10–1000 mg/mL) against S. aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Candida albicans, and Aspergillus nige r by MIC method using Mueller-Hinton broth and Sabouraud dextrose broth mediums. The results revealed that E. amoenum seed oil raised the growth of A. niger and S. epidermidis, but it inhibits the growth of P. aeruginosa and C. albicans; however, it did not show any effect on S. aureus. Trypanocidal activity of E. amoenum against Trypanosoma cruzi was investigated, and minimum lethal concentration was calculated. The results showed that different extract of E. amoenum did not show any significant activity against epimastigotes of T. cruzi.
In a study by Asadollahi et al., photoimmunological properties of E. amoenum in bovine neutrophils (polymorphonuclear neutrophil [PMN]) were tested to determine the E. amoenum effect on bovine neutrophils photoredox and phagocytosis by in vitro model system. Bovine PMN was isolated from healthy dairy cows which were preincubated with E. amoenum extract, and the impact on phagocytosis-dependent and -independent cellular chemiluminescence, phagocytosis, fluorescence-backed PMN H2O2 production, and necrosis was tested. The findings showed that phagocytosis and killing of Escherichia coli and S. aureus by PMN which cured with E. amoenum were higher than control PMN.
In a study by Nadi et al., the ability of E. amoenum extract on secretion of TNF-α by noninfected and infected mouse macrophage was evaluated by ELISA technique. At the end of the study, concentration of TNF-α in noninfected and infected macrophage culture which cured with various concentrations of E. amoenum extract was prominently higher than the control.
Effect of E. amoenum on cellular or humoral immune response was tested by lymphocyte proliferation assay and two-way mixed lymphocyte reaction. The results revealed that E. amoenum did not show stimulatory activity on either lymphocytes or thymocytes in proliferation assay, but the extract at concentration of 50–400 μg/mL had a costimulatory effect on mitogenic lymphocyte proliferation.
Antioxidant assays such as free radical scavenging, reducing power, and total antioxidant activity were carried out for ethanol, methanol, acetone, 80% methanol, and 80% ethanolic extracts. Antioxidant activity of different extracts of E. amoenum was examined by 2,2-diphenyl-1-picrylhydrazyl and reducing power assay. E. amoenum showed the highest antioxidant activity in hot water extract (100°C) and water extract (30°C). The highest and least activity was observed in water and acetone extract, respectively, in all assays.
In another study, anthocyanin-rich extract from the petals of E. amoenum (25–1000 μg/mL) was tested for total antioxidant capacity. Results revealed that the extract significantly decreased intra- and extra-cellular hydroperoxides concentration and increased ferric reducing antioxidant power value in both intra- and extra-cellular fluid at different concentration range.
Genotoxicity and cytotoxicity
Cytoprotective and antioxidant effect of E. amoenum was tested on human vascular endothelial cells under H2O2-induced oxidative stress. In conclusion, E. amoenum at the concentration of 100–1000 μg/mL decreased the cell death.
The probable effect of E. amoenum extract on DNA of hepG2 cells was assessed using the comet assay. The result showed genotoxicity of E. amoenum at the concentration of 25 μg/mL.
In a study by Gohari et al., E. amoenum extract was tested to evaluate its cytotoxicity on Salina artemia Larvae, by Brine shrimp cytotoxicity bioassay. This study revealed moderate cytotoxicity effect of E. amoenum.
Different extracts of E. amoenum were evaluated for anxiolytic effect using rotarod model of motor coordination and elevated plus maze (EPM) models. The hydroalcoholic extract did not show any significant effect on motor coordination. The anxiolytic effect was most evident in group received 125 mg/kg of aqueous extract, but the maximal efficacy of the extract is lower than diazepam. According to this study, single administration of the aqueous extract of E. amoenum produces a significant but mild-to-moderate anxiolytic effect.,
In another study, it was shown that the extract of E. amoenum increased the percentage of time spent and arm entries in the open arms of the EPM and decreased the percentage of time spent in the closed arms of EPM with dose 50 mg/kg. Furthermore, it prolonged the latency to sleep induced by ketamine without significant effect on total sleeping time. The locomotors' activity was affected by extract less than diazepam. Therefore, it seems that E. amoenum was lower than effect induced by diazepam.,
The anxiolytic effect of E. amoenum was investigated during 15-day and 30-day courses. It was shown that in 30-day treatment course, time spent in open arms was significantly higher than that of 15-day treatment in both diazepam and extract groups, and diazepam group showed the highest effect. Hence, time spent in open arms of plus maze increased depending on the duration of treatment.,
Tolerance to anxiolytic effect of E. amoenum was evaluated using light/dark box and EPM model acutely and chronically (7 days). The results showed that administration of E. amoenum significantly increased the time in the illuminated zone, without any tolerance to anxiolytic effect of the extract after 7 days.
In this research, effect of methanolic extract of E. amoenum against picrotoxin-induced seizure in mice was evaluated. The latency of seizure was increased in groups that pretreated with different doses of extract and this effect was only significant at the dose of 6.25 mg/kg. This dose delayed the death time and decreased the percentage of mortality significantly.
In a study by Hosseini and Abolhassani, both aqueous and alcoholic extracts of E. amoenum were used for the treatment of Leishmania major infection in BALB/C mice. The results showed that both extracts possessed immunomodulatory properties and increased the level of interferon-gamma as well as decreased the parasite burden in the proximal lymph nodes and prevented the necrosis of the foot pad as compared with the untreated infected mice.
Effect on cerebral ischemia
Protective effect of E. amoenum total anthocyanin extract (ETAE) on partial/transient cerebral ischemia in the rats was evaluated. The findings of this study revealed that ETAE has protective effects against cerebral ischemia, especially at the higher doses, and improved spontaneous activity and memory induced by cerebral ischemia compared to the control group. Furthermore, brain myeloperoxidase activity was decreased following cerebral ischemia. However, ETAE could not affect the ability to climbing, body proprioception, vibrissae touch, and brain water content.
Effect on blood pressure and heartbeat
Effect of aqueous extracts of E. amoenum and Citrus aurantifolia (as decoction in different doses) was studied in the regulation of blood pressure and heartbeat in rats. Heartbeat was measured noninvasively by tail-cuff method before and after phenylephrine infusion. The results showed that E. amoenum elevated blood pressure, especially in rats that had high blood pressure. Furthermore, combination of decoction of E. amoenum and C. aurantifolia was suggested for decrease of heart beats.
Effect on wound healing
The ability of E. amoenum extract ointment on wound-healing process was investigated in rats. Rats were divided to E. amoenum 1.5% ointment, eucerin-vaseline, and control groups and treated with topical ointments daily for 21 days. The findings revealed that wound size of the test groups was reduced early as compared to control and placebo groups and demonstrated that borage extract was capable of promoting wound-healing process.
Effect of E. amoenum on blood glucose, lipid profile, and lipoproteins was evaluated in streptozotocin-induced diabetic rats within 30 days. The findings of this study showed significant decrease in serum glucose, cholesterol, triglyceride, and low-density lipoprotein in effective doses. Furthermore, high-density lipoprotein level was significantly increased compared to diabetic control group.
The analgesic effect of the extract of E. amoenum was studied using formalin and hot plate test. The findings revealed that dose 10 mg/kg of the extract showed the highest analgesic effect as compared to the control group. Pretreatment of animal with naloxone before extract decreased the analgesia induced by extract. Hence, the opioids receptor may be involved partly in the analgesic effect of E. amoenum extract.
Effect on acute pancreatitis
The protective effect of E. amoenum extract on a murine model of pancreatitis was evaluated. The results of the study showed that pretreatment with E. amoenum extract reduced the inflammatory response in acute pancreatitis induced by cerulein. This study showed that E. amoenum extract decreases the severity of cerulein-induced acute pancreatitis with an anti-inflammatory, immunomodulatory, and antioxidant effect.
Hepatotoxicity effect of E. amoenum extract was studied in rats. Liver functions were assessed by serum biochemistry tests including aspartate aminotransferase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and histopathological assessment of the liver section. Serum levels of AST and ALP were reduced significantly in both 1-week and 2-week treated groups compared to controls. Reduction of ALT was observed only in 2-week treated groups.
In another study, rats were administrated by oral gavages of E. amoenum decoction for 28 days and serums were collected for liver function tests (ALT, AST, ALP, and total bilirubin). In addition, liver was isolated for histopathological study. There was no significant difference between experimental and control groups in all tests (P > 0.05) and the histopathological studies of livers showed no evidence of hepatotoxicity. The results of these studies suggest that E. amoenum has no hepatotoxicity.
Renal and hepatic effects of E. amoenum and Valeriana officinalis were investigated in animal model. The results showed significant variation of the levels of AST, ALT, and ALP in comparisons with control groups. ALP was increased significantly after oral administration of two extracts with dose of 100 and 200 mg/kg. ALT was decreased with a dose of 100 mg/kg of E. amoenum but increased only with dose of 200 mg/kg.
Effect on cataract
Prevention of sodium selenite-induced cataractogenesis by the extract of E. amoenum was evaluated in rats. In this study, protective effect of E. amoenum extract on selenite-induced cataract was significantly observed in rats and it may due to antioxidant activity of E. amoenum.
There are four clinical trials on E. amoenum aimed to evaluate the efficacy of this plant in anxiety and depression [Table 2].
The efficacy and tolerability of E. amoenum aqueous extract in combination with selective serotonin reuptake inhibitors were examined in patients with generalized anxiety disorders who met the Diagnostic and Statistical Manual of Mental Disorders-IV-TR criteria. The results revealed that E. amoenum may have positive effects on the anxiety and the positive effects start from the 2nd week. In addition, there are no serious side effects accompanying with E. amoenum.,,
In another clinical trial, antioxidant activity of E. amoenum aqueous extract was assessed in healthy subjects. Blood lipid peroxidation level was reduced significantly, and total antioxidant capacity of blood and total thiol molecule were increased after 14 days of consumption. Hence, E. amoenum has antioxidative stress potential which may be due to antioxidant components (rosmarinic acid and flavonoids).,,
Efficacy of E. amoenum in the treatment of mild-to-moderate major depression B was evaluated. Patient's assessment was done by Hamilton rating scale for depression and the Hamilton rating scale for anxiety (HAM-A14). E. amoenum aqueous extract reduced depressive symptoms significantly compares to placebo in the 4th week, but the effect on anxiety was not significant. Some side effects such as headache, somnolence, vomiting, dry mouth, constipation, and blurred vision are reported in E. amoenum group.,, Another study investigated the efficacy and safety of an aqueous extract of E. amoenum in the treatment of obsessive–compulsive disorder. Assessment of patients was done by the Yale-Brown obsessive compulsive and the HAM-A14. The results showed positive effects of extract of E. amoenum on obsession and compulsion and generalized anxiety and this effect starts from the 4th week. In addition, the results showed that use of E. amoenum has no serious side effects.
| Discussion|| |
Medicinal herbs were used as complementary medicine for many years, and they were the origins of many drugs for the treatment of diseases.
E. amoenum (Boraginaceae) is a wild biennial or perennial herb that grows in Iran. It has been used in Iranian traditional medicine for conditions such as depression and melancholy. Due to wide usage of this plant among Iranian people (especially in the form of decoction) and lack of comprehensive review on this plant, in this study, we presented a comprehensive review of chemical composition, pharmacological studies, and clinical evaluations of E. amoenum.
Chemical composition of essential oil and extract of E. amoenum was studied in different articles.
The main component of volatile oil was sesquiterpenes. Some pyrrolizidine alkaloids, phenolic compounds, cyaniding, and anthocyanidin were isolated from the extract of E. amoenum.,In vitro and in vivo studies of E. amoenum showed different biological activities such as antimicrobial, antiviral, antioxidant, analgesic, antidiabetic, anticonvulsant, and immunomodulatory activity and also anxiolytic effect of this plant.,,,,,, In addition, the extract of E. amoenum showed significant effect in acute pancreatitis with no hepatotoxicity effect.
Different chemical compounds of E. amoenum can be responsible for biological activities of it. For example, rosmarinic acid has some biological activities such as antioxidative, antiviral, and anti-inflammatory activities. Furthermore, cyanidin glycosides showed antioxidant, anti-inflammatory, and antidiabetic effects.,,,,
Clinical studies of E. amoenum in the form of capsule showed positive effect of this plant on anxiety and mild-to-moderate depression B.
The results of this review showed that E. amoenum possessed different biological activities. Some of these studies confirmed the traditional therapeutic effects of E. amoenum which were mentioned in Iranian traditional texts. According to wide consumption of this plant in Iranian folk medicine and limited clinical trials, it suggested that other therapeutic effects and effective doses of E. amoenum were investigated using well-designated clinical studies. Indeed, determination of the active components of this plant and synthesis of new effective drugs are recommended for future studies.
This study was supported by School of Persian Medicine and Research Institute for Islamic and Complementary Medicine, Iran University of Medical Sciences, Tehran, Iran.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Nadi F. Development of a new model for mass transfer kinetics of petals of Echium amoenum
Fisch. and C.A. Mey. Under fluidized bed conditions. Food Technol Biotechnol 2016;54:217-27.
Mozaffarian D. A Dictionary of Iranian Plant Names. Vol. V. Tehran: Farhang Moaser; 1996.
Ranjbar A, Khorami S, Safarabadi M, Shahmoradi A, Malekirad AA, Vakilian K, et al
. Antioxidant activity of Iranian Echium amoenum
Fisch and CA Mey flower decoction in humans: A cross-sectional before/after clinical trial. Evid Based Complement Altern Med 2006;3:469-73.
Amin GR. Popular Medicinal Plants of Iran. Vol. 1. Tehran: Iranian Research Institute of Medicinal Plants; 1991.
Mikaili P, Shayegh J, Asghari MH. Review on the indigenous use and ethnopharmacology of hot and cold natures of phytomedicines in the Iranian traditional medicine. Asian Pac J Trop Biomed 2012;22 Suppl: S1189-93.
Shafaghi B, Naderi N, Tahmasb L, Kamalinejad M. Anxiolytic effect of Echium amoenum
L. in mice. Iran J Pharm Res 2010:37-41.
Heidari MR, Azad EM, Mehrabani M. Evaluation of the analgesic effect of Echium amoenum
Fisch and C.A. Mey. extract in mice: Possible mechanism involved. J Ethnopharmacol 2006;103:345-9.
Khorasani A, Makhzan-Al-Advieh MH. Tehran, Iran.: Research Institute for Islamic and Complementary Medicine Press; 2008.
Jafari A, Amin G, Ziarati P. Potential of Echium ameonum
Fisch and Mey in removing heavy metals from pharmaceutical effluent. Biosci Biotechnol Res Asia 2016;13:1585-94.
BBekhradnia S, Ebrahimzadeh MA. Antioxidant activity of Echium amoenum
. Rev Chimie 2016;67:223-6.
Shojaii A, Fard MA. Review of pharmacological properties and chemical constituents of Pimpinella anisum
. ISRN Pharm 2012;2012:510795.
Abdollahi Fard M, Shojaii A. Efficacy of Iranian traditional medicine in the treatment of epilepsy. Biomed Res Int 2013;2013:692751.
Shojaii A, Ghods R, Fard MA. Medicinal herbs in Iranian traditional medicine for learning and memory. Afr J Tradit Complement Altern Med 2016;13:199-209.
Amirghofran Z. Medicinal plants as immunosuppressive agents in traditional iranian medicine. Iran J Immunol 2010;7:65-73.
Abolhassani M. Antibacterial effect of borage (Echium amoenum
) on Staphylococcus aureus
. Braz J Infect Dis 2004;8:382-5.
Sayyah M, Siahpoosh A, Khalili H, Malayeri A, Samaee H. A double-blind, placebo-controlled study of the aqueous extract of Echium amoenum
for patients with general anxiety disorder. Iran J Pharm Res 2012;11:697-701.
Ghassemi N, et al
. Volatile constituents of a medicinal plant of Iran, Echium amoenum
Fisch. and C.A. Mey. Daru 2003;11:32-3.
Mehrabani M, Ghannadi A, Sajjadi E, Ghassemi N, Shams-Ardakani M. Toxic pyrrolizidine alkaloids of Echium amoenum
Fisch. and Mey. Daru 2006;14:122-7.
Behnammanesh G, Khalilpour S, Majid ASA, Majid AMSA. Pharmacological actions and potential neuroprotective effects of Rhus coriaria
L. and Echium amoenum
L.: A brief review. 2015.
Mehrabani M, Ghassemi N, Sajjadi E, Ghannadi A, Shams-Ardakani M. Main phenolic compound of petals of Echium amoenum
Fisch. and C.A. Mey., a famous medicinal plant of Iran. Daru 2005;13:65-9.
Adel Pilerood S, Prakash J. Evaluation of nutritional composition and antioxidant activity of borage (Echium amoenum
) and Valerian
). J Food Sci Technol 2014;51:845-54.
Abolhassani M. Antiviral activity of borage (Echium amoenum
). Arch Med Sci 2010;6:366-9.
Farahani M. Antiviral effect assay of aqueous extract of Echium amoenum
-L against HSV-1. Zahedan J Res Med Sci 2013;15:46-8.
Mansouri S. Inhibition of Staphylococcus aureus
mediated by extracts of Iranian plants. Pharm Biol 1999;37:375-7.
Bonjar GS. Evaluation of antibacterial properties of Iranian medicinal plants against Micrococcus luteus, Serratia marcescens, Klebsiella pneumoniae
and Bordetella bronchoseptica
. Asian J Plant Sci 2004;3:82-6.
Hamedi J, Vatani M. Antibacterial and antifungal effects of evening primrose. Nova Biol Reperta 2015;2:199-206.
Etebari M, Zolfaghari B, Jafarian-Dehkordi A, Rakian R. Evaluation of DNA damage of hydro-alcoholic and aqueous extract of Echium amoenum
and Nardostachys jatamansi
. J Res Med Sci 2012;17:782-6.
Asadollahi F, Mehrzad J, Chaichi MJ, Taghavi Razavizadeh A. Photoimmunological properties of borage in bovine neutrophil in vitro
model. J Photochem Photobiol B 2015;151:270-5.
Nadi M, Mosaffa N, Karimi F, Kamalinejad M, Farrokhi B, Anissian A, et al.
Iranian black tea and cowslip extracts induce tumor necrosis factor-alpha secretion from mouse macrophage cell culture. Iran J Pharm Res 2010;9:83-7.
Amirghofran Z, Azadbakht M, Keshavarzi F. Echium amoenum
stimulate of lymphocyte proliferation and inhibit of humoral antibody synthesis. Irn J Med Sci 2000;25:119-24.
Safaeian L, Haghjoo Javanmard S, Ghanadian M, Seifabadi S. Cytoprotective and antioxidant effects of Echium amoenum
anthocyanin-rich extract in human endothelial cells (HUVECs). Avicenna J Phytomed 2015;5:157-66.
Gohari AR, Saeidnia S, Gohari MR, Moradi-Afrapoli F, Malmir M, Hadjiakhoondi A, et al
. Brine shrimp cytotoxicity of some medicinal plants belongs to Lamiaceae, Asteraceae, Rosaceae
families. J Med Plants 2009;8:87-93.
Mehrabani M, Mehrabani M, Raftari S, Nabipour F, Heidary MR, Mahdavi Z, et al
. Evaluation of hepatotoxicity of common doses of decoction of Echium amoenum
Fisch and CA Mey in rats. J Kerman Univ Med Sci 2007;14:44-54.
Saki K, Bahmani M, Rafieian-Kopaei M. The effect of most important medicinal plants on two important psychiatric disorders (anxiety and depression)-a review. Asian Pac J Trop Med 2014;7S1:S34-42.
Zahedi MJ, Heidari M, Mohajeri M. Study the effect of Valeriana officinalis
and Echium amoenum
on the liver and renal function tests in rats. J Kerman Univ Med Sci 2004;11:22--7.
Rabbani M, Sajjadi SE, Vaseghi G, Jafarian A. Anxiolytic effects of Echium amoenum
on the elevated plus-maze model of anxiety in mice. Fitoterapia 2004;75:457-64.
Noroozpour Dailami K, Azadbakht M, Lashgari M, Rashidi Z. Prevention of selenite-induced cataractogenesis by hydroalchoholic extract of Echium amoenum
: An experimental evaluation of the Iranian traditional eye medication. Pharm Biomed Res 2015;1:40-7.
Hasani-Ranjbar S, Larijani B, Abdollahi M. A systematic review of the potential herbal sources of future drugs effective in oxidant-related diseases. Inflamm Allergy - Drug Targets. 2009;8:2-10.
Gholamzadeh S, Zare S, Ilkhanipoor M. Anxiolytic effect of Echium amoenum
during different treatment courses. Res J Biol Sci 2008;3:176-8.
Sarris J, Camfield D, Berk M. Complementary medicine, self-help, and lifestyle interventions for obsessive compulsive disorder (OCD) and the OCD spectrum: A systematic review. J Affect Disord 2012;138:213-21.
Rabbani M, Sajjadi SE, Khalili S. A Lack of tolerance to the anxiolytic action of Echium amoenum. Res Pharm Sci 2011;6:101-6.
Heidari MR, Mandegary A, Hosseini A, Vahedia M. Anticonvulsant effect of methanolic extract of Echium amoenum Fisch and C.A. Mey. against seizure induced by picrotoxin in mice. Pakistan Journal of Biological Sciencce 2006;9:772-6.
Hosseini, N, Abolhassani M. Immunomodulatory properties of borage (Echium amoenum
) on BALB/c mice infected with Leishmania major
. J Clin Immunol 2011;31:465-71.
Safaeian L, Tameh AA, Ghannadi A, Naghani EA, Tavazoei H, Alavi SS, et al.
Protective effects of Echium amoenum
Fisch. And C.A. Mey. Against cerebral ischemia in the rats. Adv Biomed Res 2015;4:107.
Hamidi EM, Khaksari M, Hojabri K. The effects of aqueous extracts of Echium amoenum
and citrus aurantiflia on blood pressure and heart rate before and after phenylephrine injection in rat. J Kerman Univ Med Sci 2011;18:349-57.
Farahpour MR, Mavaddati AH. Effects of borage extract in rat skin wound healing model, histopathological study. J Med Plants Res 2012;6:651-6.
Mahmoudi M, Shahidi S, Golmohammadi H, Mohammadi S. he effect of Echium amoenum
hydro-alcoholic extract on blood glucose level, lipid profile and lipoproteins in streptozotocin-induced diabetic male rats. J Zanjan Univ Med Sci Health Serv 2015;23:72-81.
Abed A, Minaiyan M, Ghannadi A, Mahzouni P, Babavalian MR. Effect of Echium amoenum
Fisch. Et Mey a traditional Iranian herbal remedy in an experimental model of acute pancreatitis. ISRN Gastroenterol 2012;2012:141548.
Zamansoltani F, Nassiri-Asl M, Karimi R, Mamaghani-Rad P. Hepatotoxicity effects of aqueous extract of Echium amoenum
in rats. Pharmacologyonline 2008;1:432-8.
Khanahmadi M, Rezazadeh S. Review on Iranian medicinal plants with antioxidant properties. J Med Plants 2010;9:19-32.
Boostani H, Saiiah M, Fazileh F, Kamalinejad M, Akhondzadeh S. Efficacy of aqueous extract of Echium amoenum
L. in the treatment of mild to moderate obsessive–compulsive disorder. J Med Plants 2005;3:43-50.
Dwyer AV, Whitten DL, Hawrelak JA. Herbal medicines, other than st. John's wort, in the treatment of depression: A systematic review. Altern Med Rev 2011;16:40-9.
Sarris J, Panossian A, Schweitzer I, Stough C, Scholey A. Herbal medicine for depression, anxiety and insomnia: A review of psychopharmacology and clinical evidence. Eur Neuropsychopharmacol 2011;21:841-60.
Ahmad R, Ahmad N, Naqvi AA, Shehzad A, Al-Ghamdi MS. Role of traditional Islamic and Arabic plants in cancer therapy. J Tradit Complement Med 2017;7:195-20.
Abbaszadeh S, Radjabian T, Taghizadeh M, Fazeli F, Salmaki Y. Characterization of fatty acids in different organs of some Iranian Echium
plants. J Med Plants Res 2011;5:4814-21.
Zhu F, Asada T, Sato A, Koi Y, Nishiwaki H, Tamura H, et al.
Rosmarinic acid extract for antioxidant, antiallergic, and α-glucosidase inhibitory activities, isolated by supramolecular technique and solvent extraction from perilla leaves. J Agric Food Chem 2014;62:885-92.
Jin BR, Chung KS, Cheon SY, Lee M, Hwang S, Noh Hwang S, et al
. Rosmarinic acid suppresses colonic inflammation in dextran sulphate sodium (DSS)-induced mice via dual inhibition of NF-kappaB and STAT3 activation. Sci Rep 2017;7:46252.
Sarris J, McIntyre E, Camfield DA. Plant-based medicines for anxiety disorders, Part 2: A review of clinical studies with supporting preclinical evidence. CNS Drugs 2013;27:301-19.
Saiiah Bargard M, Assadi SM, Amini H, Saiiah M, Akhondzadeh S, Kamalinejad M. Efficacy of aqueous extract of Echium amoenum
L. in the treatment of mild to moderate major depressive disorder: A randomized double blind clinical trial. J Med Plants 2004;3:61-8.
[Table 1], [Table 2]