Home | About us | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |  Login 
Pharmacognosy Reviews
Search Article 
Advanced search 
Year : 2017  |  Volume : 11  |  Issue : 21  |  Page : 1-7

Review of angiotensin-converting enzyme inhibitory assay: Rapid method in drug discovery of herbal plants

1 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Mulawarman University, Samarinda, East Kalimantan 75116; Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Indonesia, Indonesia
2 Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Indonesia, Indonesia
3 Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java 16424, Indonesia

Correspondence Address:
Abdul Mun'im
Faculty of Pharmacy, Universitas Indonesia, Building A, 3rd Floor, Rumpun Ilmu Kesehatan, Kampus UI Depok, West Java 16424
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/phrev.phrev_45_16

Rights and Permissions

The renin-angiotensin-aldosterone system is a signaling pathway which responsible in the blood pressure regulation. Angiotensin-converting enzyme (ACE) is one of the key elements responsible for the hypertensive mechanism. It converts angiotensin-I to angiotensin-II. The discovery history of the ACE inhibitory activity assay method has been through a long stage for decades and development continues until today. The ACE inhibitory activity has become an effective screening method in the search for new antihypertensive agents from herbal plants. Some of in vitro assay methods were used to examine the activity of ACE inhibitors based on the substrate usage, such as; Cushman and Cheung Method using a substrate hippuryl-histidyl-leucine (HHL), Holmquist method using a substrate furanacryloyl-tripeptide, Elbl and Wagner method using a substrate benzoil-[l-14C] glicyl-L-histidine-L-leucine, Carmel and Yaron method using a substrate o-aminobenzoylglycyl-p-nitrophenylalanilproline, and Lam method using 3-hydroxybutyrylglycyl-glycyl-glycine as substrate. Several different methods to measure the results of enzymatic reactions or separating substrate with products, including spectrophotometric, fluorometric, high-performance liquid chromatography, electrophoresis, and radiochemistry. Application of the test method for screening the ACE inhibitors activity and investigation of active compounds from natural products can be done easily with this method, it is very helpful in research because the results obtained are simple, accurate, and rapid.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded9    
    Comments [Add]    

Recommend this journal