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REVIEW ARTICLE
Year : 2011  |  Volume : 5  |  Issue : 10  |  Page : 189-194  

Artemisinin biosynthesis and its regulatory enzymes: Progress and perspective


Department of Natural Medicinal Chemistry, Jinan University, Guangzhou, China

Date of Submission27-Mar-2011
Date of Decision19-Jul-2011
Date of Web Publication23-Dec-2011

Correspondence Address:
Rongmin Yu
College of Pharmacy, Jinan University, Guangzhou 510632
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0973-7847.91118

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   Abstract 

Artemisinin is an endoperoxidized sesquiterpene from the Chinese medicinal plant Artemisia annua, used as an effective anti-malarial drug. Its biosynthesis pathway has been investigated for many years for scientific interest and for potential manufacturing applications to fulfill the market demand. A number of regulatory enzymes of its biosynthesis process, including amorpha-4,11-diene synthase, CYP71AV1 and cytochrome P450 reductase, have been obtained and utilized to increase the content of artemisinin. However, a large knowledge gap still exists, and certain points of controversy have stirred debates within the field. In this review, the progress and perspective of artemisinin biosynthesis and its regulating enzymes are described.

Keywords: Artemisinin, biosynthesis pathway, progress and perspective, regulatory enzymes


How to cite this article:
Wen W, Yu R. Artemisinin biosynthesis and its regulatory enzymes: Progress and perspective. Phcog Rev 2011;5:189-94

How to cite this URL:
Wen W, Yu R. Artemisinin biosynthesis and its regulatory enzymes: Progress and perspective. Phcog Rev [serial online] 2011 [cited 2017 May 30];5:189-94. Available from: http://www.phcogrev.com/text.asp?2011/5/10/189/91118


   Introduction Top


Artemisinin

Malaria is a leading cause of epidemic and death worldwide with an estimated number of 400 million infections and 1-3 million deaths per year. [1] The disease occurs due to the mosquito-mediated infection of erythrocytes by the malarial parasite Plasmodium falciparum. [2] Young children and pregnant women are most vulnerable to contracting this disease. The anti-malarial properties of the traditional Chinese medicine qinghaosu (artemisinin) were discovered by Chinese scientists in 1971 from Artemisia annua. [3] Although before the late 1960s, quinine-derived agents controlled malaria efficiently, multi-drug resistant Plasmodium strains have made artemisinin the preferred treatment due to its rapid therapeutic efficacy and low toxicity.

Artemisinin is a sesquiterpene lactone with an unusual endoperoxide structure. Numerous derivatives of artemisinin have also been synthesized and tested against malaria parasites, [4] many of which are derivatives at C 12 . During recent years, a great number of artemisinin analogs have been synthesized, such as trioxolane OZ 277, which was advanced into clinical studies during 2004. [5] This trioxolane compound was orally administered and highly potent, but its development was discontinued prior to phase 3 clinical trials because of its instability in blood. [3] Thus, the need for additional anti-malarial drugs remains immensely urgent.

Artemisinin specifically and selectively inhibits the sarco/endoplasmic reticulum Ca 2+ ATPase (SERCA) of P. falciparum after activation by iron ions. [6] Its specific mechanism is still unclear but the necessity of the endoperoxide bridge for antimalarial activity has been largely established. Besides the anti-malarial activity, artemisinin and its derivatives have exhibited other activities as well, such as anti-schistosomal, [7] anti-viral [8] and anti-cancer activities. [9]

Artemisinin-derived drugs typically have a short half-life and are best used in combination with other anti-malarials such as lumefantrine and sulfadoxime/pyrimethamine in artemisinin-based combination therapies (ACTs). [10] Artemisinin-based combination therapies (ACTs) recommended by WHO (2001) are highly effective in preventing the infection and transmission of MDR malaria. [2]

An estimated number of 400-600 million therapeutic doses of artemisinin will be required for ACT per year, whereas less than 100 million doses per year are presently available. [1] The demand of ACTs has caused artemisinin to fall in short supply. Furthermore, artemisinin on the market is currently produced only from A. annua, which contains only small amounts of artemisinin at about 0.01% to 0.8%. [11] The yield of artemisinin often depends on additional variable, such as temperature, humidity and soil type, resulting in high market prices for artemisinin range from US $350 to $1700 per kilogram. [3] Consequently, tremendous efforts have been made to increase the production of artemisinin. However, the knowledge about the biosynthetic pathway of artemisinin is not directly lacking.

Biosynthetic pathway of artemisinin

The biosynthesis pathway of artemisinin has been investigated for many years, but the whole process is not completely understood. Overall, two stages can be recognized in the biosynthesis of regular monoterpenes, sesquiterpenes and diterpenes, which involves the production of linear isoprene precursors including geranyl pyrophosphate (GPP), farnesyl pyrophosphate (FPP) and geranylgeranyl-pyrophosphate (GGPP), and the formation of cyclic terpenes from linear isoprene precursors. [2] The enzymes responsible for the first stage are conserved across all organisms, while the second stage is catalyzed by enzymes that vary from species to species.

Formation of FPP and enzymes

For decades, the classic cytoplasmic mevanolate (MVA) pathway had been considered as the only pathway for terpenes biosynthesis, which was first discovered by Bloch and Lynen in 1958. It has two isoprenoid precursors, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), which are biosynthesized via mevalonate originating from acetyl-CoA. However, another biosynthetic pathway was elucidated recently. This plastidic non-MVA pathway, or deoxyxylulose phosphate (DXP)/methylerythritol phosphate (MEP) pathway, stems from glyceric acid-3-phosphate and pyruvate. Initial research indicated that IPP of mevalonate origin served as a precursor of farnesyl diphosphate (FPP), and ultimately, of sesquiterpenes, triterpenes and sterols, whereas the non-mevalonate route provided the precursors of geranyl diphosphate (GPP), geranylgeranyl-diphosphate (GGPP) and ultimately, of monoterpenes, diterpenes and carotenoids. [1] However, more and more evidence now suggests that crosstalk coexists between these two pathways for the biosynthesis of FPP.

Young A. annua plants were treated with specific chemical inhibitors of MVA or non-MVA pathway separately, after which the formation of artemisinin was found to decrease in both groups. [12] It demonstrated that precursor IPP was provided not only by the MVA pathway but also by the non-MVA pathway. Recently, a possible scenario is brought forward and supported: DMAPP of mevalonate origin is transferred to the plastid, where an IPP unit of non-mevalonate origin is used for elongation affording geranyl diphosphate (GPP). In the subsequent step, GPP is exported to the cytosolic compartment and converted into FPP using IPP from the mevalonate pathway. [1] The whole formation process of FPP via MVA and non-MVA pathways is shown in [Figure 1].
Figure 1: The formation of FPP via both MVA and non-MVA pathways and regulatory enzymes

Click here to view


The formation of FPP is catalyzed by a series of enzymes. In the MVA pathway, ATOT (acetoacetyl-CoA thiolase), HMGS (3-hydroxyl-3-methyglutaryl CoA synthase), HMGR (3-hydroxyl-3-methyglutaryl CoA reductase), MK (mevalonate kinase), MPK (mevalonate-5-phosphate kinase), MPD (mevalonate pyrophosphate decarboxylase) and IPI (IPP isomerase) all play important roles, while in the non-MVA pathway, enzymes like DXS (1-deoxy-D-xylulose-5-phosphate synthase), DXR (1-deoxy-D-xylulose-5-phosphate reductoisomerase), CMS (4-diphosphocytidyl-2C-methyl-D-erythritol 4-phosphate synthase), CMK (4-diphosphocytidyl-2-C-methyl-D-erythritol kinase), MCS (2-C-methyl-D- erythritol 2,4-cyclodiphosphate synthase), HDS (1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase), IDS (IPP/DMAPP synthase) are involved.

HMGR is significant by catalyzing HMG-CoA to MVA, and considered as the first rate-limiting enzyme in MVA pathway, given that MVA formation is an irreversible process. Genome or cDNA cloning products of HMGR in tomato, potato, Arabidopsis thaliana, etc. have been reported in the early days, and in A. annua till 1995, cloned by Kang. Genes of HMGR and FPPS were co-expressed in transgenic A. annua plants, inducing 2.32-fold content increase of artemisinin. [13] Experiments in vivo modulated HMGR activity to increase artemisinin biosynthesis through exogenous supply of labeled HMG-CoA, and proved that plant growth regulators, IAA and GA 3 , enhanced the artemisinin accumulation by increasing the activity of HMGR. [14] On the contrary, it is also supported by Re et al that HMGR does not limit the rate of terpenes biosynthesis in some plant cells.

DXS catalyzed the first step in non-MVA pathway, promoting the formation of DXP from pyruvate and glyceric acid-3-phosphate, serving as an essential rate-limiting enzyme. DXR is another rate-limiting enzyme in non-MVA pathway, which is an effective target for regulating terpene biosynthesis. The exogenous supply of a competitive inhibitor of DXR, fosmidomycin, inhibited 14.2% of artemisinin accumulation. [14] Furthermore, it has been reported that DXS transcript accumulation was substantially increased when the root cultures of A. annua were grown in light as compared to the roots grown in dark. [15]

The sequential head-to-tail condensations of DMAPP and IPP consequently form the linear prenyl diphosphate precursors, GPP and FPP, which are catalyzed by the prenyltransferases: GPP synthase (GPPS) and FPP synthase (FPPS), respectively. [2] These enzymes belong to prenyltransferase (PTS). A cDNA encoding FPPS has been cloned from a cDNA library of A. annua, and expressed in  Escherichia More Details coli cells, whose deduced amino acid sequence was highly similar to that of FPPS from yeast and mammals. Since FPP is the key precursor in the pathway to artemisinin, genetic manipulation of FPPS becomes promising. This suggestion was proven by over-expression of the endogenous FPPS in high-yield A. annua; the highest artemisinin content in transgenic A. annua was up to 0.9% DW and 34.4% higher than that in non-transgenic A. annua. [16] Expression of FPPS was observed in transgenic A. annua, exhibiting higher artemisinin content and yield of 2.5- and 3.6-fold, respectively, than that detected in wild plants. [17]

As of now, the biosynthesis of FPP from aceyl-CoA is clear, and a large progress in understanding the process from FPP to artemisinic acid has made. However, how artemisinic acid transforms into artemisinin is still under investigation.

Characteristic pathway of artemisinin biosynthesis and the involved enzymes

Sesquiterpene synthases

A number of cDNAs from A. annua encoding different sesquiterpene synthases have been revealed. The cDNA encoding sesquiterpene synthase firstly cloned belongs to the epi-cedol synthase (ECS), followed by the genetic cloning of amorpha-4,11-diene synthase (ADS) [18] and β-caryophyllene synthase (CS).[19] After that, scientists reported the cloning of an (E)-β-farnesene synthase (FS) from A. annua in [Figure 2]. [20]
Figure 2: Reactions catalyzed by the four sesquiterpene synthases cloned from A. annua

Click here to view


ECS: Epi-cedol synthase; ADS: Amorpha-4,11-diene synthase; CS: β-caryophyllene synthase; FS: (E)-β-farnesene synthase.[20]

All the above-mentioned sesquiterpene synthases have been cloned from A. annua leaves. However, to investigate the biosynthesis of sesquiterpenes, Bertea et al. firstly isolated total RNA from the glandular trichomes and used it for the construction of a plasmid cDNA library. Thus, a new sesquiterpene synthase, a germacrene A synthase, from a glandular trichome-specific cDNA library of A. annua was reported. [21]

The first committed and limiting step of artemisinin biosynthesis should be the cyclization of FPP by ADS. Therefore, the up-regulation of amorphar-4,11-diene synthase is considered a feasible strategy for increasing the production of artemisinin in A. annua. ADS gene was cloned and expressed in A. annua, resulting in a 2.3-fold increase in artemisinin content compared to non-transgenic A. annua. [22] Recently, several groups have cloned some new cDNA of ADS from different strains of A. annua. A newly cloned ADS1 cDNA from a special high-yield strain of A. annua was reported by Li et al., which is not completely identical to ADS that has been cloned by others after molecular and phylogenetic analysis. [23] Besides, the ADS (3963) gene was obtained from high artemisinin yielding strain of A. annua, which was studied by an in silico approach. [24] Over-expression of these genes may be possible ways to accumulation of artemisinin.

ADS accumulations in leaves are 16-fold higher than in roots and 10-fold higher than in stems, demonstrating a tissue-specific expression pattern. In general, ADS content remains low in the cells under normal conditions. Interestingly, the flowering field plants and cold-acclimated cultural plants produce higher levels of ADS than non-flowering field plants or untreated cultural plants, indicating the environmental and developmental induction on ADS. [25] It is consistent with the experiments that upon exposure to cold, heat shock or UV light, the transcription levels of ADS were up-regulated compared to untreated plants. [26]

Since squalene synthase (SS) is responsible for sterol biosynthesis, competitive with FPP, it is considered as a regulatory switch. An antisense SS cDNA was introduced into the genome of A. annua, resulting in the decline of SS mRNA and simultaneous reduction of total sterols, mRNA coding for amorpha-4,11-diene synthase tremendously elevated. [27]

Cytochrome P 450

Cytochrome P 450 enzymes (CYP/P 450 ) with dehydrogenase and reductase activities were assumed to participate in the oxidation of C 12 in amorpha-4,11-diene. Moreover, a cDNA clone encoding a cytochrome P 450 designated CYP71AV1 was characterized by expression in Saccharomyces cerevisiae and shown to catalyze the oxidation of the amorpha-4,11-diene, artemisinic alcohol and artemisinic aldehyde, [28] as shown in [Figure 3]. Indeed, when co-expressed in yeast with genes required for amorphadiene biosynthesis, artemisinic acid was formed in culture. [4]
Figure 3: Biosynthesis pathway of artemisinin and enzymes

Click here to view


Note : Steps confirmed are shown in bold arrows.

Expression analysis of CYP71AV1 in A. annua tissues indicates that it is most highly expressed in trichomes, moderately expressed in flower buds and lowly expressed in leaves and roots. [28] Zeng et al reported that CYP71AV1 accumulates in leaves is eight-fold higher than in roots and four-fold than in stems. Similar with ADS, CYP71AV1 genes is up-regulated to 10- and 7-fold in cultured A. annua after being induced by low temperature stress. [25] It is supported by Yin et al. that the transcription levels of CYP71AV1 were tremendously enhanced in stress conditions, and artemisinin content increased by 66.7-95.6% in transient pre-chilling plants than untreated plants. [26]

CPR (cytochrome P 450 reductase), as the companion body of CYP71AV1, has been cloned and co-expressed with CYP71AV1 and ADS in S. cerevisiae, leading to high levels of artemisinic acid. [29] Different from ADS and CYP71AV1, CPR seemed to keep an average transcription level either with or without stress. [26]

Artemisinic aldehyde ∆11(13) reductase

It is obvious that the ∆11(13) double bond in amorpha-4,11-diene is reduced during biosynthesis of artemisinin, which is assumed to occur in artemisinic aldehyde. Recently, a corresponding gene Dbr2 was cloned and characterized, shown to be relatively specific for artemisinic aldehyde and some activity on small α, β-unsaturated carbonyl compounds. Expression in yeast of Dbr2 and other enzymes of artemisinin pathway in yeast resulted in the accumulation of dihydroartemisinic acid.[30] A chemotype of A. annua with a high artemisinin level typically has high ratios of dihydroartemisinic acid to artemisinic acid, [31] which indicates that double bond reduction may limit artemisinin accumulation. [32]

Aldehyde dehydrogenase

As mentioned above, cytochrome P 450 CYP71AV1 was shown to be capable of oxidizing artemisinic alcohol to the corresponding aldehyde and acid. However, the ability of CYP71AV1 oxidizing dihydroartemisinic aldehyde was not detected. A full-length cDNA encoding an aldehyde dehydrogenase homolog was isolated and named Aldh1, which was capable of catalyzing the oxidation artemisinic aldehyde, dihydroartemisinic aldehyde and a limited range of other aldehydes. In addition, the highest expression of Aldh1 in A. annua was observed in the glandular trichomes with moderate expression in the flower buds and low expression in the leaves. [33]

Other possible intermediates and enzymes

The entire biosynthetic pathway from amorpha-4,11-diene to artemisinin remains uncertain. The mainly controversial issue on latest steps in artemisinin biosynthesis is whether artemisinic or dihydroartemisinic acid serves as the later precursor.

Artemisinic acid is a common precursor of arteannuin B and artemisinin. Artemisinin was formed after artemisinic acid and arteannuin B incubation with A. annua leaves extract, respectively. Scientists purified and characterized an enzyme involved in biochemical transformation of arteannuin B to artemisinin from A. annua. [34] However, genes of this enzyme have not been reported. The evidence above suggests the possibility of artemisinin biosynthesis from artemisinic acid. Furthermore, a full-length cDNA (apod1) encoding a peroxidase was isolated from A. annua, which favored the bioconversion of artemisinic acid to artemisinin in the cell-free extract of A. annua indirectly but no oxidation with artemisinic acid as the only substrate. [35] It has been reported that the level of artemisinin from artemisinic acid was elevated to about one-fold when the horseradish peroxidase was included in cell-free extracts of A. annua by phosphate buffer system. [36]

Recently, Brown et al have reported that artemisinic acid was converted in vivo, possible non-enzymatically into several compounds including arteannuin B, but not artemisinin. In addition, in vivo dihydroartemisinic acid undergoes rapid plant pigment photosensitized oxidation, followed by subsequent spontaneous oxidation to form artemisinin. [37] Dihydroartemisinic acid can act as a quencher of the singlet oxygen to produce dihydroartemisinic acid hydroperoxide, which is later transformed into artemisinin. [38] Notably, perhaps the least spontaneous step in dihydroartemisinic acid oxidation, first singlet oxygen-dependent hydroperoxidation and resulting ring cleavage is very reminiscent of other enzyme reactions, like carotenoid cleavage dioxygenases, which catalyze the cleavage of double bonds to give two carbonyl compounds. Taking into account of above ideas, whether artemisinin biosynthesis from dihydroartemisinic acid involves enzymes is an open question and worthy of being further studies.

Experimental results showed that there was no direct enzymatic conversion of artemisinic acid into dihydroartemisinic acid. [39] Therefore, these two branches during artemisinin biosynthesis remain controversial as shown in [Figure 3].


   Conclusion Top


The study of artemisinin biosynthesis has made a tremendous progress, especially after a number of regulating enzymes were cloned, isolated and characterized. Since 1995, 12 genes related to artemisinin biosynthesis have been cloned from A. annua and their complete or partial mRNA sequences are now accessible in GenBank. Since plant materials are affected by natural environment, and direct extraction is laborious, more and more attentions are now shifting to microbial production. Microbe-based artemisinin production can avoid contamination by other plant-made terpenes, thereby simplifying processing steps for purification. [2] Additionally, with the development of technology, genetic regulation, as a popular method, is adopted all over the world to increase artemisinin production. Consequently, the elucidation of artemisinin biosynthesis pathway is much needed. However, there are still some problems which should be resolved in the future. Any improvements or new discovery of artemisinin biosynthesis are likely to lower the price and bring great benefits to millions around the world.

 
   References Top

1.Schramek N, Wang HH, RÖmisch-Margl W, Keil B, Radykewicz T, WinzenhÖrlein B, et al. Artemisinin biosynthesis in growing plants of Artemisia annua. a 13 CO 2 study. Phytochemistry 2010;71:179-87.  Back to cited text no. 1
    
2.Zeng Q, Qiu F, Yuan L. Production of artemisinin by genetically-modifiedmicrobes. Biotechonol Lett 2008;30:581-92.  Back to cited text no. 2
    
3.White NJ. Qinghaosu (Artemisinin) The price of success. Science 2008;320:331-4.  Back to cited text no. 3
    
4.Covello PS, Teoh KH, Polichuk DR, Reed DW, Nowak G. Functional genomics and the biosynthesis of artemisinin. Phytochemistry 2007;68:1864-71.  Back to cited text no. 4
    
5.Vennerstrom JL, Arbe-Barnes S, Brun R, Charman SA, Chiu FC, Chollet J, et al. Identification of an antimalarial synthetic trioxolane drug development candidate. Nature 2004;430:900-4.  Back to cited text no. 5
    
6.Eckstein-Ludwig U, Webb RJ, Goethem IDA, East JM, Lee AG, Kimura M, et al. Artemisinins target the SERCA of Plasemodium falciparum. Nature 2003;424:957-61.  Back to cited text no. 6
    
7.Xiao SH. Development of antischistosomal drugs in China, with particular consideration to Praziquantel and the Artemisinins. Acta Trop 2005;96:153-67.  Back to cited text no. 7
    
8.Romero MR, Serrano MA, Vallejo M, Efferth T, Alvarez M, Marin JJ. Antiviral effect of artemisinin from Artemisia annua against a model member of the Flaviviridae family, the bovine viral diarrhea virus (BVDV). Planta Med 2006;72:1169-74.  Back to cited text no. 8
    
9.Efferth T. Molecular pharmacology and pharmacogenomics of artemisinin and its derivatives in cancer cells. Curr Drug Targets 2006;7:407-21.  Back to cited text no. 9
    
10.Mutabingwa TK. Artemisinin-based combination therapies (ACTs): Best hope for malaria treatment but inaccessible to the needy. Acta Trop 2005;95:305-15.  Back to cited text no. 10
    
11.Abdin MZ, Israr M, Rehman RU, Jain SK. Artemisinin, a novel antimalarial drug: Biochemical and molecular approaches for enhanced production. Planta Med 2003;69:289-99.  Back to cited text no. 11
    
12.Towler MJ, Weathers PJ. Evidence of artemisinin production from IPP stemming from both the mevalonate and the nonmevalonate pathways. Plant Cell Rep 2007;26:2129-36.  Back to cited text no. 12
    
13.Tang KX, Jing FY, Zhang L, Wang GF. Method for increasing artemisinin content by co-transferring genes HMGR and FPS into Artemisia annua [P]. Faming Zhuanli Shenqing 2008;CN 101182545A .  Back to cited text no. 13
    
14.Ram M, Khan MA, Jha P, Khan S, Kiran U, Ahmad MM, et al. HMG-CoA reductase limits artemisinin biosynthesis and accumulation in Artemisia annua L. Plants. Acta Physiol Plant 2010;32:859-66.  Back to cited text no. 14
    
15.Souret FF, Kim Y, Wyslouzil BE, Wobbe KK, Weathers PJ. Scale-up of Artemisia annua L. hairy root cultures produces complex patterns of terpenoid gene expression. Biotechnol Bioeng 2003;83:653-67.  Back to cited text no. 15
    
16.Han JL, Liu BY, Ye HC, Wang H, Li ZQ, Li GF. Effects of overexpression of the endogenous farnesyl diphosphate synthase on the artemisinin content in Artemisia annua L. J Integrat Plant Biol 2006;48:482-7.  Back to cited text no. 16
    
17.Banyai W, Kirdmanee C, Mii M, Supaibulwatana K. Overexpression of farnesyl pyrophosphate synthase gene affected artemisinin content and growth of Artemisia annua L. Plant Cell Tiss Organ 2010;103:255-65.  Back to cited text no. 17
    
18.Mercke P, Bengtsson M, Bouwmeester HJ, Posthumus MA, Brodelius PE. Molecular cloning, expression and characterization of amorpha-4,11-diene synthase, a key enzyme of artemisinin biosynthesis in Artemisia annua L. Arch Biochem Biophys 2000;381:173-80.  Back to cited text no. 18
    
19.Cai Y, Jia JW, Crock J, Lin ZX, Chen XY, Croteau R. A cDNA clone for â-caryophyllene synthase from Artemisia annua. Phytochemistry 2002;61:523-9.   Back to cited text no. 19
    
20.Picaud S, Brodelius M, Brodelius PE. Expression, purification and characterization of recombinant (E)-â-farnesene synthase from Artemisia annua. Phytochemistry 2005;66:961-7.  Back to cited text no. 20
    
21.Bertea CM, Voster A, Verstappen FW, Maffei M, Beekwilder J, Bouwmeester HJ. Isoprenoid biosynthesis in Artemisia annua: Cloning and heterologous expression of a germacrene a synthase from a glandular trichome cDNA library. Arch Biochem Biophys 2006;448:3-12.   Back to cited text no. 21
    
22.Tang KX, Jing FY, Zhang L, Wang GF. Transformation of gene ADS into Artemisia annua to increase arteannuin content [P]. Faming Zhuanli Gongkai Shuomingshu 2008;CN 101182544A.   Back to cited text no. 22
    
23.Li ZQ, Liu Y, Liu BY, Wang H, Ye HC, Li GF. Cloning, E. coli expression and molecular analysis of amorpha-4,11-diene synthase from a high-yield strain of Artemisia annua L. J Integrat Plant Biol 2006;48:1466-92.  Back to cited text no. 23
    
24.Alam P, Kiran U, Ahmad MM, Kamaluddin, Khan MA, Jhanwar S, et al. Isolation, characterization and structural studies of amorpha-4,11-diene synthase (ADS(3963)) from Artemisia annua L. Bioinformation 2010;4:421-9.   Back to cited text no. 24
    
25.Zeng QP, Zeng XM, Yin LL, Yang RY, Feng LL, Yang XQ. Quantification of three key enzymes involved in artemisinin biogenesis in Artemisia annua by polyclonal antisera-based ELISA. Plant Mol Biol Rep 2009;27:50-7.   Back to cited text no. 25
    
26.Yin L, Zhao C, Huang Y, Yang RY, Zeng QP. Abiotic stress-induced expression of artemisinin biosynthesis genes in Artemisia annua L. Chin J Appl Environ Biol 2008;14:1-5.  Back to cited text no. 26
    
27.Feng LL, Yang RY, Yang XQ, Zeng XM, Lu WJ, Zeng QP. Synergistic re-channeling of mevalonate pathway for enhanced artemisinin production in transgentic Artemisia annua. Plant Sci 2009;177:57-67.  Back to cited text no. 27
    
28.Teoh KH, Polichuk DR, Reed DW, Nowak G, Covello PS. Artemisia annua L. (Asteraceae) trichome-specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin. FEBS Lett 2006;580:1411-6.  Back to cited text no. 28
    
29.Ro DK, Paradise EM, Ouellet M, Fisher KJ, Newman KL, Ndungu JM, et al. Producation of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature 2006;440:940-3.  Back to cited text no. 29
    
30.Zhang YS, Teoh KH, Reed DW, Maes L, Goossens A, Olson DJ, et al. The molecular cloning of artemisinic aldehyde ∆11(13) reductase and its role in glandular trichome-dependent biosynthesis of artemisinin in Artemisia annua. J Biol Chem 2008;283:21501-8.  Back to cited text no. 30
    
31.Wallaart TE, Pras N, Beekman AC, Quax WJ. Seasonal variation of artemisinin and its biosynthetic precursors in plants of Artemisia annua of different geographical origin: Proof for the existence of chemotypes. Planta Med 2000;66:57-62.  Back to cited text no. 31
    
32.Covello PS. Making artemisinin. Phytochemistry 2008;69:2881-5.  Back to cited text no. 32
    
33.Teoh KH, Polichuk DR, Reed DW, Covello PS. Molecular cloning of an aldehyde dehydrogenase implicated in artemisinin biosynthesis in Artemisia annua. Botany 2009;87:635-42.  Back to cited text no. 33
    
34.Dhingra V, Narasu ML. Purification and characterization of an enzyme involved in biochenmical transformation of arteannuin B to artemisinin from Artemisia annua. Biochem Bioph Res Co 2001;281:558-61.  Back to cited text no. 34
    
35.Zhang YS, Liu BY, Li ZQ, Ye HC, Wang H, Li GF, et al. Molecular cloning of a classical plant peroxidase from Artemisia annua and its effect on the biosynthesis of artemisinin in vitro. Acta Bot Sin 2004;46:1338-46.  Back to cited text no. 35
    
36.Zhang YS, Ye HC, Li GF. Effect of horseradish peroxidase on the biosynthesis of artemisinin in Artemisia annua in vitro. Chin J Appl Envirom Biol 2003;9:616-8.   Back to cited text no. 36
    
37.Brown GD, Sy LK. In vivo transformations of artemisinic acid in Artemisia annua plants. Tetrahedron 2007;63:9548-66.  Back to cited text no. 37
    
38.Brown GD, Sy LK. In vivo transformations of diartemisinic acid in Artemisia annua plants. Tetrahedron 2004;60:1139-59.  Back to cited text no. 38
    
39.Bertea CM, Freije JR, Van der Woude H, Verstappen FW, Perk L, Marquez V, et al. Identification of intermediates and enzymes involved in the early steps of artemisinin biosynthesis in Artemisia annua. Planta Med 2005;71:40-7.  Back to cited text no. 39
    


    Figures

  [Figure 1], [Figure 2], [Figure 3]


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[Pubmed] | [DOI]
5 Transcriptome analysis of Thapsia laciniata rouy provides insights into terpenoid biosynthesis and diversity in apiaceae
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